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Safety Data Sheet (SDS) APC Anti-mouse/human CD207 Langerin  Antibody     Product Data Sheet (PDF)    
APC Anti-mouse/human CD207 (Langerin) Antibody
1321025 25 µg $110.00       
1321030 100 µg $290.00       
Clone: 4C7
Isotype: Mouse IgG2a, κ
Reactivity: Mouse, Human, Cross-Reactivity: Cynomolgus, Rabbit, Swine (Pig, Porcine)
Immunogen: Langerin extracellular domain-Fc fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Cells from a collagenase-digested BALB/c

Cells from a collagenase-digested BALB/c mouse spleen were stained with CD11c Alexa Fluor® 488 and CD207 (clone 4C7) APC (top) or mouse IgG2a, κ APC isotype control (bottom).





Description:

CD207, also known as langerin, is a 40 kD single-pass type II transmembrane protein, member of the C-type lectin family. CD207 is expressed on the cell surface and Birbeck granules (BGs) of Langerhans cells, and subsets of thymic and splenic dendritic cells. Its ligands are mannose, n-acetylglucosamine, fucose, and sulfated glycans. Langerin is involved in the antigen processing pathway through capture and internalization of its ligands.

Other Names: CLEC4K
Structure: Single-pass type II transmembrane protein, 40 kD, one C-type lectin domain, homotrimer
Distribution: Langerhans cells, subsets of thymic and splenic dendritic cells
Function: Antigen uptake, antigen processing
Ligand Receptor: Mannose, n-acetylglucosamine, fucose, sulfated glycans
Antigen References:

1. Romani N, et al. 2012. Curr. Top. Microbiol. Immunol. 351:113.
2. Kaplan DH. 2010. Trends Immunol. 31:446.
3. Clausen BE and Kel JM. 2010. Immunol. Cell. Biol. 88:351.
4. Merad M, et al. 2008. Nat. Rev. Immunol. 8:935.