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Safety Data Sheet (SDS) Alexa Fluor 647 Anti-mouse CD85k Gp49 Receptor Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 647 Anti-mouse CD85k (gp49 Receptor) Antibody
1324530 100 µg $0.00       
1324525 25 µg $120.00       
Clone: H1.1
Isotype: Armenian Hamster IgG
Reactivity: Mouse
Immunogen: gp49B-Fc fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Clone H1.1 recognizes both gp49A and gp49B. Additional reported applications (for the relevant formats) include in vitro activation of gp49B to inhibit cytokine production. 

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Application References:

1. Wang LL, et al. 2000. J. Immunol. 164:5215. (Activ)

C57BL/6 mouse bone marrow cells

C57BL/6 mouse bone marrow cells were stained with CD11b PE and CD85k (clone H1.1) Alexa Fluor® 647 or Armenian hamster IgG Alexa Fluor® 647 isotype control (bottom).





Description:

gp49, also known as ILT3, gp49b1 or CD85k, is a 49 kD glycoprotein expressed on activated NK cells and myeloid cells. It structurally resembles killer inhibitory receptors (KIR). gp49B is a type I membrane protein belonging to the Ig superfamily and contains an immunoreceptor tyrosine-based inhibitory motif (ITIM), while gp49A is a truncated molecule without ITIM. Upon activation following interaction with integrin avb3, the cytoplasmic tail associates with SHP-1 and SHP-2, which results in downregulation of cell activation and cytokine production.  

Other Names: Leukocyte immunoglobulin-like receptor, subfamily B, member 4 (Lilrb4), gp49, gp49B1, gp49B, ILT3
Structure: Type I membrane protein belonging to Ig superfamily
Distribution: Myeloid cells, activated NK cells
Function: Inhibitory activity mediated by ITIM motif
Ligand Receptor: Integrin αvβ3, collagen
Interaction: SHP-1 and SHP-2
Antigen References:

1. Castells, MC, et al. 1994. J. Biol. Chem. 269:8393.
2. Katz HR, et al. 1996. P. Natl. Acad. Sci. USA 93:10809.
3. Kuroiwa A, et al. 1998. J. Biol. Chem. 273:1070.
4. Wang LL, et al. 2000. J. Immunol. 164:5215.
5. Jiang L, et al. 2009. Eur. J. Immunol. 39:1167.


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