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Safety Data Sheet (SDS) Biotin Anti-mouse CD185 CXCR5 Antibody     Product Data Sheet (PDF)    
Biotin Anti-mouse CD185 (CXCR5) Antibody
1327545 25 µg $90.00       
1327550 100 µg $255.00       
Clone: L138D7
Isotype: Rat IgG2b, κ
Reactivity: Mouse
Immunogen: mCXCR5-transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Clone L138D7 staining works optimally at room temperature or 4°C. Unlike other chemokine receptor antibodies, avoid using L138D7 at 37°C.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Kim Yu, et al. 2015. PLoS One. 10:120294. PubMed

C57BL/6 mouse splenocytes were stained

C57BL/6 mouse splenocytes were stained with CD45R/B220 FITC and biotinylated CXCR5 (clone L138D7, top) or rat IgG2b, κ isotype control (bottom), followed by Sav-PE.





Description:

CD185 is also known as CXCR5. It is the receptor for chemokine CXCL13/BLC, which is chemotactic for B cells.  CXCR5 is expressed on B cells and a subset of T cells in the spleen, neuronal tissue, lymph nodes, and bone marrow.  It is important for migration of B cells into the B cell follicles of the spleen and Peyer’s patches.  Follicular helper T cells (Tfh) also express CXCR5 and the ability of these cells to migrate to the lymph node is modulated by the balanced expression of CCR7 and CXCR5.

Other Names: BLR1, GPCR6, MDR-15
Structure: 42 kD, seven transmembrane protein
Distribution: B cells and a subset of T cells in mouse spleen, Tfh cells, also expressed in lymph node, bone marrow, and brain
Function: B cell migration into follicles, possible regulatory role in B cell differentiation and Burkitt's lymphoma
Ligand Receptor: BLC/CXCL13
Antigen References:

1. Kaiser E, et al. 1993. Eur. J. Immunol. 23:2532.
2. Forster R, et al. 1994. Cell. Mol. Biol. 40:381.
3. Forster R, et al. 1994. Blood 84:830.
4. Forster R, et al. 1996. Cell 87:1037.
5. Wolf I, et al. 1998. J. Biol. Chem. 273:28831.