Sony Biotechnology Inc. Sony Biotechnology Inc.cart
About Us Products What's New Library News Calendar Contact Us Place Order
Safety Data Sheet (SDS) PE/Cy7 Anti-mouse CD107a LAMP-1 Antibody     Product Data Sheet (PDF)    
PE/Cy7 Anti-mouse CD107a (LAMP-1) Antibody
1208095 25 µg $105.00       
1208100 100 µg $285.00       
Clone: 1D4B
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: This monoclonal antibody was raised against NIH/3T3 mouse embryo fobroblast tissue culture cell membranes. It has been mapped to the N-terminus of LAMP-1.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Cy7 under optimal conditions. The solution is free of unconjugated PE/Cy7 and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC, ICFC - Quality tested

Application Notes:

This antibody is effective in immunoblotting (WB) and immunohistochemistry (IHC).

* Expected MW: 120 kD
WB Positive Control: Mouse 3T3 cell lysate and Mouse Spleen
IHC Positive Control: Mouse Pancreas, Colon, Kidney

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Thioglycollate-elicited BALB/c mouse peritoneal macrophages

Thioglycollate-elicited BALB/c mouse peritoneal macrophages were surface stained with CD107a (clone 1D4B) PE/Cy7 (filled histogram) or rat IgG2a, κ PE/Cy7 isotype control (open histogram).

Thioglycollate-elicited BALB/c mouse peritoneal macrophages

Thioglycollate-elicited BALB/c mouse peritoneal macrophages were intracellularly stained with CD107a (clone 1D4B) PE/Cy7 (filled histogram) or rat IgG2a, κ PE/Cy7 isotype control (open histogram).



Description:

CD107a, also known as Lysosome-Associated Membrane Protein 1 (LAMP-1) or LGP-120, is a 110-140 kD type I membrane glycoprotein. Mature CD107a is heavily glycosylated from a 40 kD core protein. This molecule is located on the luminal side of lysosomes. Upon activation, CD107a is transferred to the cell membrane surface of activated platelets, activated lymphocytes, macrophages, epithelial cells, endothelial cells, and some tumor cells. CD107a has been suggested to play a role in the protection of lysosomal membrane from lysosomal hydrolases which is involved in cell adhesion and regulation of tumor metastasis, and mediates autoimmune disease progression. CD107a is a ligand for galaptin and E-selectin. Surface expression of LAMP-1 has been shown to correlate with CD8+ T cell and NK cell cytotoxicity.

Other Names: Lysosome-associated membrane glycoprotein 1, lysosomal membrane glycoprotein 1, CD107 antigen-like family member A, lysosome-associated membrane protein 1

This product or portions thereof is manufactured under license from GE Healthcare under U.S. Patent Numbers 5,268,486; 5,569,587; 5,627,027 and patents or pending applications that are continuations, continuations-in-part, re-examinations, divisionals, reissues or foreign equivalents thereof. THIS MATERIAL IS SUBJECT TO PROPRIETARY RIGHTS OF GE HEALTHCARE BIO-SCIENCES CORP. AND CARNEGIE MELLON UNIVERSITY AND MADE AND SOLD UNDER LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. THIS PRODUCT IS LICENSED FOR SALE ONLY FOR RESEARCH. IT IS NOT LICENSED FOR ANY OTHER USE. THERE IS NO IMPLIED LICENSE HEREUNDER FOR ANY COMMERCIAL USE.
COMMERCIAL USE shall include:

  • sale, lease, license or other transfer of the material or any material derived or produced from it;
  • sale, lease, license or other grant of rights to use this Material or any material derived or produced from it;
  • use of this material to perform services for a fee for third parties.

IF YOU REQUIRE A COMMERCIAL LICENSE TO USE THIS MATERIAL AND DO NOT HAVE ONE RETURN THIS MATERIAL, UNOPENED TO Sony Biotechnology Inc., 2100 South Oak Street, Champaign, IL 61820, USA AND ANY MONEY PAID FOR THE MATERIAL WILL BE REFUNDED.