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Safety Data Sheet (SDS) PE Anti-human/mouse Bcl-6 Antibody     Product Data Sheet (PDF)    
PE Anti-human/mouse Bcl-6 Antibody
2392515 25 tests $120.00       
2392520 100 tests $320.00       
Clone: 7D1
Isotype: Rat IgG2a, κ
Reactivity: Human, Mouse
Immunogen: Sequence 226-398 of murine BLC6 fused with GST
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

ICFC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: Western blotting1,2,3.

Recommended Usage:

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining using our nuclear factor staining protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Vikstrom, et al. 2010. Science 330:1095. (WB)
2. Kallies A, et al. 2011. Blood 117:1869. (WB)
3. Lüthje K, et al. 2012. Nat. Immunol. 13:491. (WB)
4. Tonti E, et al. 2012. J. Immunol. 188:3217. (ICFC)

11-day immunized C57BL/6 mouse lymph

11-day immunized C57BL/6 mouse lymph node cells were surface stained with B220 Pacific Blue™ and IgD PerCP/Cy5.5, then fixed and permeabilized with Nuclear Factor Fixation and Permeabilization Buffer Set. Cells were then stained with Bcl-6 (clone 7D1) PE (top) or rat IgG2a, κ PE isotype control (bottom). Data shown was gated on B220+ positive cells.





Description:

B-cell lymphoma 6 (Bcl-6), is an 80 kD homodimer, member of the BTB-POZ zinc finger family. It contains 1 BTB (POZ) domain and 6 C2H2-type zinc fingers. Bcl-6 is a transcriptional repressor, master regulator of germinal center reaction. On B cells, Bcl-6 induces proliferation, antibody class switch and affinity maturation, while inhibits its differentiation to plasma cells. On T cells, Bcl-6 induces its differentiation to TFH. This molecule is also expressed in some B cell lymphomas and breast cancer cells.

Other Names: B-cell lymphoma 6, BCL5, LAZ3, BCL6A, ZNF51, ZBTB27
Structure: Member of the BTB-POZ zinc finger family, 80 kD, homodimer, 1 BTB (POZ) domain, 6 C2H2-type zinc fingers
Distribution: Germinal center B cells, follicular helper T cells (TFH), B cell lymphomas, breast cancer cells
Function: Transcription factor, master regulator of germinal center reaction
Interaction: BLIMP-1
Antigen References:

1. Basso K and Dalla-Favera R. 2012. Immunol. Rev. 247:172.
2. Vinuesa CG, and Cyster JG, 2011. Immunity 35:671.
3. Kitano M, et al. 2011. Immunity 34:961.
4. Baumjohann D, et al. 2011. J. Immunol. 187:2089.
5. Johnston RJ, et al. 2009. Science. 325:1006.
6. Tran TH, et al. 2010. Cancer Res. 70:1711.