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Safety Data Sheet (SDS) Purified Anti-mouse CLEC2 CLEC1B Antibody     Product Data Sheet (PDF)    
Purified Anti-mouse CLEC2 (CLEC1B) Antibody
1330510 100 µg $205.00       
Clone: 17D9/CLEC-2
Isotype: Rat IgG2b, κ
Reactivity: Mouse
Immunogen: RBL-2H3 cells stably expressing HA-tagged mouse CLEC2.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application:

FC - Quality tested
WB - Reported in the literature

Application Notes:

Additional reported applications (for relevant formats) include: Western blotting1.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Mourão-Sá D, et al. 2011. Eur. J. Immunol. 41:3040. (WB)

C57BL/6 mouse platelets were stained

C57BL/6 mouse platelets were stained with purified CLEC-2 (clone 17D9/CLEC-2) (filled histogram) or purified rat IgG2b, κ isotype control (open histogram), followed by anti-rat IgG PE.



Description:

CLEC-2, also known as CLEC1B and CLEC1B1, is C-type lectin-like receptor with a single cytoplasmic YXXL sequence known as a hem-immunoreceptor tyrosine-based activation motif (hemITAM). CLEC-2 is highly expressed on resting platelets and megakaryocytes and at a lower levels on several hematopoietic cells including monocytes, macrophages, dendritic cells, and granulocytes. The first ligand that was identified for CLEC-2 was the snake venom toxin rhodocytin; podoplanin has also been identified as an endogenous ligand for CLEC-2. Exposure of platelets to rhodocytin leads tyrosine phosphorylation of the ITAM-like motif and Syk-dependent platelet activation. CLEC-2 also functions as an activation receptor on monoyctes and neutrophils to induce phagocytosis and proinflammatory cytokine production.

Other Names: CLEC1B, CLEC1B1
Structure: Type II transmembrane protein with a single cytoplasmic YXXL sequence known as a hem-immunoreceptor tyrosine-based activation motif (hemITAM)
Distribution: Platelets, megakaryocytes, granulocytes, monocytes, macrophages, and dendritic cells
Function: Involved in platelet aggregation and secretion as well as neutrophil phagocytosis
Ligand Receptor: Venom toxin, rhodocytin, and podoplanin
Antigen References:

1. Boulaftali Y, et al. 2013. J. Clin. Invest. 123:908. 
2. Manne BK, et al. 2013. J. Biol. Chem. 288:7717.
3. Acton SE, et al. 2012. Immunity. 37:276. 
4. Finney BA, et al. 2012. Blood 119:1747. 
5. Kerrigan AM, et al. 2009. J. Immunol. 182:4150.