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Safety Data Sheet (SDS) Purified Annexin V     Product Data Sheet (PDF)    
Purified Annexin V
3804505 100 µg $205.00       
3804510 500 µg $620.00       
Reactivity: All mammalian species
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The protein was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The Annexin V solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Annexin V Staining 
1.
2. Transfer 100 µl of cell suspension in 5 ml test tube.
3. Add 5 µl of purified Annexin V.
4. Add 10 µl of PI solution or 7-AAD . 
5. Gently vortex the cells, and incubate for 15 min at room temperature (25°C), in the dark. 
6. Add 400 µl of Annexin V Binding Buffer to each tube. Analyze by flow cytometry.

Recommended Usage:

Each lot of this product is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric application, the suggested use of this reagent is 5 - 15 µg per 100,000 - million cells in a 100 µl volume of Annexin V Binding Buffer (Cat No. 422201). It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Koopman G, et al. 1994. Blood 84:1415. 
2. Vermes I, et al. 1995. J. Immunol. Methods 184:39. 
3. Dachary-Prigent J, et al. 1993. Blood 81:2554.
4. Sekine C, et al. 2009. Int Immunol. PubMed
5. Grujic M, et al. 2010. J. Immunol. 185:1730. PubMed

Human T cell leukemia cell

Human T cell leukemia cell line, Jurkat, was treated (4 hours) with (top 2 panels) or without (bottom two panels) LEAF™ purified anti-CD95 (clone EOS9.1), then stained with Annexin V FITC (panels 1 & 3) or with purified recombinant Annexin V to block Annexin V FITC staining (panels 2 & 4).





Description:

Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner. PS is normally only found on the intracellular leaflet of the plasma membrane in healthy cells, but during early apoptosis, membrane asymmetry is lost and PS translocates to the external leaflet. Fluorochrome-labeled Annexin V can then be used to specifically target and identify apoptotic cells. Annexin V Binding Buffer is recommended for use with Annexin V staining. Annexin V binding alone cannot differentiate between apoptotic and necrotic cells. Therefore, we recommend using our 7-AAD Viability Staining Solution or Propidium Iodide Solution . Early apoptotic cells will exclude 7-AAD and PI, while late stage apoptotic cells and necrotic cells will stain positively, due to the passage of these dyes into the nucleus where they bind to DNA.

Other Names: Annexin A5