Sony Biotechnology Inc. Sony Biotechnology Inc.cart
About Us Products What's New Library News Calendar Contact Us Place Order
Safety Data Sheet (SDS) Purified Anti-human/mouse/rat CD205 DEC-205 Antibody     Product Data Sheet (PDF)    
Purified Anti-human/mouse/rat CD205 (DEC-205) Antibody
2396010 100 µg $250.00       
Clone: HD83
Isotype: Mouse IgG1, κ
Reactivity: Human, Mouse, Rat, Cross Reactivity: Monkey
Immunogen: hDEC205-hIgG1Fc protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application:

FC - Quality tested
IHC, WB - Reported in the literature

Application Notes:

Additional reported applications (for relevant formats) include: immunohistochemical staining of frozen tissue sections1 and Western blotting1.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Park CG, et al. 2012. J. Immunol. Methods 377:15. (IHC, WB)

Human peripheral blood monocyte-derived dendritic

Human peripheral blood monocyte-derived dendritic cells were stained with CD209 (clone 9E9A8, mouse IgG2a) APC and purified CD205 (clone HD83) (top) or purified mouse IgG1, κ isotype control (bottom), followed by anti-mouse IgG1 PE.





Description:

CD205, also known as DEC-205 and LY75, belongs to the macrophage mannose receptor (MMR) family of C-type lectins. Both human and mouse CD205 share multiple similar structures: an N-terminal cysteine-rich domain, a fibronectin type II domain, and a series of 10 tandemly repeated C-type like domains (CTLDs). CD205 is highly expressed on dendritic cells and thymic cortical epithelial cells in humans and mice. It also can be detected on T and B cells, monocytes, neutrophils, and epithelial cells. CD205 is a novel endocytic receptor used by dendritic cells and thymic epithelial cells to direct captured antigens from the extracellular space to specialized antigen processing areas. It mediates antigen uptake and presentation and cross-presentation to T cells. It has been reported that CD205 acts as a recognition receptor for dying cells, which potentially provides an important pathway for the uptake of self-antigen in the intrathymic environment, and is involved in peripheral tolerance.

Other Names: LY75
Structure: An N-terminal cysteine-rich domain, a fibronectin type II domain and a series of 10 tandemnly repeated C-type like domains (CTLDs)
Distribution: Expressed at high levels by dendritic cells and thymic epithelial cells; also expressed by B lymphocytes, macrophages, Langerhans cells, bone marrow cells, and epithelial cells
Function: Mediates endocytosis, antigen uptake, presentation, and cross-presentation to T cells
Antigen References:

1. Jiang W, et al. 1995. Nature 375:151.
2. Small M and Kraal G. 2003. Int. Immunol. 15:197.
3. Shrimpton RE, et al. 2009. Mol. Immunol. 46:1229.