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Safety Data Sheet (SDS) PerCP/Cy55 Anti-mouse GM-CSF Antibody     Product Data Sheet (PDF)    
PerCP/Cy5.5 Anti-mouse GM-CSF Antibody
3127045 25 µg $125.00       
3127050 100 µg $305.00       
Clone: MP1-22E9
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: Yeast-expressed, recombinant mouse GM-CSF
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cy5.5 under optimal conditions. The solution is free of unconjugated PerCP/Cy5.5 and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

ICFC - Quality tested

Application Notes:

ELISA or ELISPOT Capture1,3-5: The purified MP1-22E9 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MP1-31G6 antibody as the detecting antibody.

Recommended Usage:

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cy5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application References:

1. Sander B, et al. 1993. J. Immunol. Methods 166:201.
2. Suda T, et al. 1990. Cell. Immunol. 129:228.
3. Nozaki S, et al. 1991. J. Invest. Dermatol. 97:10.
4. Abrams JS, et al. 1992. Immunol. Rev. 127:5.
5. Abrams JS. 2001. Curr. Protoc. Immunol. Unit 6.20.
6. Sander B, et al. 1991. Immunol. Rev. 119:65.
7. Andersson U, et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag.
8. Larkin J, et al. 2006. J. Immunol. 177:268.

PMA+ Ionomycin-stimulated Th2-polarized C57BL/6 splenocytes

PMA+ Ionomycin-stimulated Th2-polarized C57BL/6 splenocytes were surface stained with CD3 FITC and intracellularly stained with GM-CSF (clone MP1-22E9) PerCP/Cy5.5 (top) or rat IgG2a, κ PerCP/Cy5.5 isotype control (bottom).





Description:

GM-CSF is a hematopoietic factor that is produced by T cells, macrophages, fibroblasts and endothelial cells. This multifunctional cytokine stimulates progenitor cells of neutrophils, eosinophils, and macrophages. GM-CSF is also a differentiation and activating factor for granulocytic and monocytic cells.

Other Names: Granulocyte/macrophage-colony stimulating factor, CSF-α, Pluripoietin-α, Eosinophil colony stimulating factor (Eo-CSF), Burst promoting activity (BPA)
Structure: Cytokine; 22 kD (Mammalian)
Regulation: Synergistic with IL-1, IL-3, G-CSF; E21R competitive antagonist for receptor binding; stored in ECM with heparan sulfate proteoglycans
Cellular Sources: T cells, monocytes/macrophages, fibroblasts, endothelial cells, mast cells
Cellular Targets: Granulocyte/macrophage/erythroid/megakaryocytic progenitors, myeloblasts, monoblasts
Receptors: Heterodimer GM-CSFR α subunit (CDw116); β-subunit (CDw131) in common with IL-3R, IL-5R
Bioactivity/Activities: Growth/development granulocyte/macrophage progenitors; differentiates myeloblasts/monoblasts; synergizes with Epo proliferation of erythroid/megakaryocytic progenitors
Antigen References:

1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Demetri, G., et al. 1991. Blood 78:2791.
3. Fan, D., et al. 1991. In vivo 5:571.
4. Negrin, R., et al. 1992. Adv. Pharmacol. 23:263.


This product or portions thereof is manufactured under license from GE Healthcare under U.S. Patent Numbers 5,268,486; 5,569,587; 5,627,027 and patents or pending applications that are continuations, continuations-in-part, re-examinations, divisionals, reissues or foreign equivalents thereof. THIS MATERIAL IS SUBJECT TO PROPRIETARY RIGHTS OF GE HEALTHCARE BIO-SCIENCES CORP. AND CARNEGIE MELLON UNIVERSITY AND MADE AND SOLD UNDER LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. THIS PRODUCT IS LICENSED FOR SALE ONLY FOR RESEARCH. IT IS NOT LICENSED FOR ANY OTHER USE. THERE IS NO IMPLIED LICENSE HEREUNDER FOR ANY COMMERCIAL USE.
COMMERCIAL USE shall include:

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