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Safety Data Sheet (SDS) PE Anti-human/mouse/rat CD205 DEC-205 Antibody     Product Data Sheet (PDF)    
PE Anti-human/mouse/rat CD205 (DEC-205) Antibody
2396015 25 tests $120.00       
2396020 100 tests $290.00       
Clone: HD83
Isotype: Mouse IgG1, κ
Reactivity: Human , Mouse, Rat, Cross Reactivity: Monkey
Immunogen: hDEC205-hIgG1Fc protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for relevant formats) include: immunohistochemical staining of frozen tissue sections1 and Western blotting1.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Park CG, et al. 2012. J. Immunol. Methods 377:15. (IHC, WB)

Human peripheral blood monocyte-derived dendritic

Human peripheral blood monocyte-derived dendritic cells were stained with CD209 APC and CD205 (clone HD83) PE (top) or mouse IgG1, κ PE isotype control (bottom).





Description:

CD205, also known as DEC-205 and LY75, belongs to the macrophage mannose receptor (MMR) family of C-type lectins. Both human and mouse CD205 share multiple similar structures: an N-terminal cysteine-rich domain, a fibronectin type II domain, and a series of 10 tandemly repeated C-type like domains (CTLDs). CD205 is highly expressed on dendritic cells and thymic cortical epithelial cells in humans and mice. It also can be detected on T and B cells, monocytes, neutrophils, and epithelial cells. CD205 is a novel endocytic receptor used by dendritic cells and thymic epithelial cells to direct captured antigens from the extracellular space to specialized antigen processing areas. It mediates antigen uptake and presentation and cross-presentation to T cells. It has been reported that CD205 acts as a recognition receptor for dying cells, which potentially provides an important pathway for the uptake of self-antigen in the intrathymic environment, and is involved in peripheral tolerance.

Other Names: LY75
Structure: An N-terminal cysteine-rich domain, a fibronectin type II domain and a series of 10 tandemnly repeated C-type like domains (CTLDs)
Distribution: Expressed at high levels by dendritic cells and thymic epithelial cells; also expressed by B lymphocytes, macrophages, Langerhans cells, bone marrow cells, and epithelial cells
Function: Mediates endocytosis, antigen uptake, presentation, and cross-presentation to T cells
Antigen References:

1. Jiang W, et al. 1995. Nature 375:151.
2. Small M and Kraal G. 2003. Int. Immunol. 15:197.
3. Shrimpton RE, et al. 2009. Mol. Immunol. 46:1229.