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Safety Data Sheet (SDS) APC Anti-mouse CD30 Antibody     Product Data Sheet (PDF)    
APC Anti-mouse CD30 Antibody
1111560 100 µg $220.00       
Clone: mCD30.1
Isotype: Armenian Hamster IgG
Reactivity: Mouse
Immunogen: Mouse CD30-mouse IgG1 fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: in vitro induction of IL-5 production1. For the most successful immunofluorescent staining results, it may be necessary to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody or biotinylated anti-Armenian hamster IgG second step, followed by SAv-PE ).

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Bowen MA, et al. 1996. J. Immunol. 156:442.
2. Punt JA, et al. 1997. J. Exp. Med. 186:1911.

Con A-stimulated (3 days) C57BL/6

Con A-stimulated (3 days) C57BL/6 mouse splenocytes were stained with CD30 (clone mCD30.1) APC (filled histogram) or Armenian hamster IgG APC isotype control (open histogram).



Description:

CD30 is a 105 kD type I transmembrane glycoprotein, also known as Ber-H2 or Ki-1. It is a member of the tumor necrosis factor receptor (TNFR) superfamily, expressed on activated T, B, and NK cells, and monocytes. CD28, in conjunction with several cytokines, regulates the expression of CD30 on activated T cells. It has been reported that cytokine production and Th1 versus Th2 development is heavily influenced by CD30-CD30 ligand (CD153) interactions.

Other Names: Ber-H2, Ki-1, TNFRSF8
Structure: TNFR superfamily, 105-120 kD
Distribution: Activated T cells, B cells and NK cells, monocytes
Function: Costimulation, regulates negative selection of thymocytes
Ligand Receptor: CD153 (CD30L)
Antigen References:

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Falini B, et al. 1995. Blood 85:1.
3. Gedrich RW, et al. 1996. J. Biol. Chem. 271:12852.