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Safety Data Sheet (SDS) Purified Anti-mouse CD6 Antibody     Product Data Sheet (PDF)    
Purified Anti-mouse CD6 Antibody
1332005 25 µg $50.00       
1332010 100 µg $90.00       
Clone: OX-129
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

C57Bl/6 splenocytes were stained with

C57Bl/6 splenocytes were stained with CD3 (clone 145-2C11) APC and purified anti-mouse CD6 (clone OX-129, top) or rat IgG2a, κ isotype control (bottom), followed by anti-rat IgG PE.





Description:

CD6, a 100 kD monomeric T cell surface glycoprotein, is a member of the scavenger receptor cysteine-rich protein superfamily. It is expressed on mouse thymocytes and splenic T cells and neurons, but not on splenic B cells. CD6 has a long cytoplasmic tail in mice, with two proline-rich domains that interact with the -SH3 domain binding sequence. CD6 binding to its ligand, CD166 (ALCAM), serves as a costimulatory molecule through its interaction with SLP-76. CD6 mediated signaling may contribute to thymocyte survival and functional avidity in mice and men.

Other Names: CD6/TP120
Structure: 130 kD type I membrane protein belonging to the scavenger receptor cysteine-rich protein superfamily
Distribution: T cells, thymocytes, neurons
Function: Interaction with CD166 induces T cell proliferation and tyrosine phosphorylation.
Ligand Receptor: CD166 (ALCAM)
Antigen References:

1. Osorio LM, et al. 1995. Cell. Immunol. 166:44.
2. Whitney G, et al. 1995. Mol. Immunol. 32:89.
3. Starling GC, et al. 1996. Eur. J. Immunol. 26:738.