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Safety Data Sheet (SDS) APC Anti-mouse CD122 IL-2Rbeta Antibody     Product Data Sheet (PDF)    
APC Anti-mouse CD122 (IL-2Rβ) Antibody
1216065 25 µg $100.00       
1216070 100 µg $250.00       
Clone: TM-β1
Isotype: Rat IgG2b, κ
Reactivity: Mouse
Immunogen: Rat T cell line expressing mouse CD122 ( IL-2Rβ)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: immunoprecipitation1, blocking of IL-2 binding1, and NK cell depletion2 in vivo.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Tanaka T, et al. 1991. J. Immunol. 147:2222.
2. Tanaka T, et al. 1993. J. Exp. Med. 178:1103.
3. Tanaka T, et al. 1992. Int. Immunol. 4:487.

C57BL/6 mouse splenocytes were stained

C57BL/6 mouse splenocytes were stained with CD122 (clone TM-β1) APC (filled histogram) or rat IgG2b, κ APC isotype control (dashed histogram).



Description:

CD122 is a 70-75 kD IL-2 receptor β chain also known as IL-2Rβ, which is also shared by the IL-15 receptor. It is constitutively expressed by NK cells and at lower levels by T cells, B cells, monocytes, and macrophages. The IL-2Rβ chain can combine with either the common γ subunit (γc, CD132) alone or with the γc subunit and the IL-2Rα subunit (CD25) to generate intermediate or high affinity IL-2 receptor complexes, respectively. CD122 expression levels can be upregulated by activation. The TM-β1 antibody does inhibit IL-2 binding to the IL-2 receptor. CD122 is expressed on murine, but not human, CD8+ Tregs involved in the maintenance of T cell homeostasis.

Other Names: IL-2 Receptor β chain, IL-2Rβ
Structure: Ig superfamily, forms high affinity IL-2 receptor with CD25 and CD132 chains or intermediate affinity receptor with CD132 alone, 70-75 kD
Distribution: T and B cells, NK cells, monocytes, macrophages
Function: Critical component of IL-2 and IL-15 signaling
Ligand Receptor: IL-2, IL-15
Antigen References:

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Minami Y, et al. 1993. Annu. Rev. Immunol. 11:245.
3. Suzuki H, et al. 1995. Science 268:1472.
4. Shi Z, et al. 2009. Eur. J. Immunol. 39:2109.