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Safety Data Sheet (SDS) Purified Anti-mouse CD184 CXCR4 Antibody     Product Data Sheet (PDF)    
Purified Anti-mouse CD184 (CXCR4) Antibody
1332510 100 µg $170.00       
Clone: L276F12
Isotype: Rat IgG2b, κ
Reactivity: Mouse
Immunogen: Mouse CXCR4-transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

C57BL/6 mouse thymocytes were stained

C57BL/6 mouse thymocytes were stained with purified CXCR4 (clone L276F12, top) or purified rat IgG2b, κ isotype control (bottom), followed by anti-rat IgG FITC and then stained with CD4 Alexa Fluor® 647.





Description:

CD184, also known as CXCR4, is a member of the G protein coupled receptor family that binds CXCL12 (SDF1). CXCR4 and CXCL12 play an important role in immune and inflammatory responses through the regulation of cell migration and growth. CXCR4 plays a crucial role in the pathogenesis of several autoimmune diseases such as atherosclerosis, rheumatoid arthritis, and wound healing. In addition, CXCR4 is the cofactor for fusion and entry of the T cell-tropic form of HIV-1.

Other Names: Fusin, LESTR, Sdf1r, Cmkar4, PB-CKR, PBSF/SDF-1
Structure: G-protein-coupled seven transmembrane receptor, 39.7 kD
Distribution: T lymphocytes, monocytes, macrophages, tissue-committed stem/progenitor cells (TCSCs), mast cells, vascular smooth muscle cells (VSMCs)
Function: Development of cardiovascular and central nervous system, hematopoiesis, and colonization of BM by fetal liver-derived hematopoietic stem cells (HSCs) during embryogenesis, homing and egress of CD34+ CXCR4+ progenitor cells from bone marrow, and their migration into peripheral tissues. Critical role in homing of cancer cells to specific metastatic sites. Possible role in the ubiquitin proteosome system.
Ligand Receptor: CXCL12 (SDF-1)
Interaction: Robo -1
Antigen References:

1. Kucia M, et al. 2005. Stem Cells 23:879.
2. Muller A, et al. 2001. Nature 410:50.
3. Saini V, et al. 2010. J. Biol. Chem. 285:15566.
4. Prasad A, et al. 2007. J. Leuko. Biol. 82:465. 
5. De Klerck B, et al. 2005. Arthritis Res. Ther. 7:R1208.
6. Rueda P, et al. 2008. PLoS One 3:e2543. 
7. Feng Y, et al. 1996. Science 272:872.