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Safety Data Sheet (SDS) Purified Anti-Tubulin Beta 3 TUBB3 Antibody     Product Data Sheet (PDF)    
Purified Anti-Tubulin Beta 3 (TUBB3) Antibody
3887005 25 µg $85.00       
3887010 100 µg $195.00       
Clone: AA10
Isotype: Mouse IgG2a, κ
Reactivity: Mouse, Rat, Human
Immunogen: Fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application:

WB - Quality tested
IF - Validated

Recommended Usage:

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. For immunofluorescent staining, the suggested use of this reagent is 1 - 5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.



Mouse brain (lane 1), mouse

Mouse brain (lane 1), mouse liver (lane 2), N-tera2 (lane 3), and NF-1 (lane 4) cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal tubulin beta 3 (TUBB3) (clone AA10) antibody. Proteins were visualized using an anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection.



Description:

Tubulin is the main component of microtubules. In adults, tubulin beta 3 (TUBB3) is primarily expressed in neurons and is commonly used as a neuronal marker. It plays an important role in neuronal cell proliferation and differentiation. Mutations in this gene cause congenital fibrosis of the type 3 extraocular muscles. Tubulin beta 3 (TUBB3) is also found in a wide range of tumors. Studies indicate that it is a predictive and prognostic marker in various tumors.

Other Names: β-3 Tubulin, Neuronal class III beta-tubulin, β3-tub
Structure: 450 amino acids with predicted molecular weight of 50 kD
Distribution: Cytosol
Function: Plays important roles in neuronal cell proliferation and differentiation
Interaction: Alpha tubulin, kinesin and dynein
Antigen References:

1. Katsetos CD, et al. 2003. J. Child Neurol. 18:851.
2. Mobarakeh ZT, et al. 2012. Cell Biol. Int. Rep. (2010) 19:e00015.
3. Locher H, et al. 2013. Differentiation. 85:173.
4. Karki R, et al. 2013. Expert Opin. Ther. Targets. 17:461.
5. Mariani M, et al. 2011. Curr. Mol. Med. 11:726.
6. Koh Y, et al. 2009. Ann. Oncol. 20:1414.