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Safety Data Sheet (SDS) Brilliant Violet 421 Anti-mouse CD144 VE-cadherin Antibody     Product Data Sheet (PDF)    
Brilliant Violet 421™ Anti-mouse CD144 (VE-cadherin) Antibody
1290065 50 µg $235.00       
Clone: BV13
Isotype: Rat IgG1, κ
Reactivity: Mouse
Immunogen: VE-cadherin-Ig fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions. The solution is free of unconjugated Brilliant Violet 421™ and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Clone BV13 recognizes an epitope between aa 45 and 56, and has a binding affinity of 5-15 nM.5 Additional reported applications (for relevant formats) include: Western blotting1, blocking of cell interactions in vivo1, and immunofluorescence microscopy4.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Application References:

1. Corada M, et al. 1999. P. Natl. Acad. Sci. USA 96:9815. (WB, Block)
2. Liao F, et al. 2000. Cancer Res. 60:6805. (FC)
3. Crosby CV, et al. 2005. Blood 105:2771. (FC)
4. Liao F, et al. 2002. Cancer Res. 62:2567. (IF)
5. May C, et al. 2005. Blood 105:4337. (epitope)

Mouse endothelial cells bEnd.3 were

Mouse endothelial cells bEnd.3 were stained with CD144 (clone BV13) Brilliant Violet 421™ (filled histogram) or rat IgG1 Brilliant Violet 421™ isotype control (open histogram).



Description:

CD144, also known as vascular endothelial-cadherin (VE-cadherin), is a 120 kD member of the type II Cadherin family.  It is an endothelial specific hemophilic adhesion molecule involved in endothelial cell survival, migration, contact-dependent growth inhibition, and homophilic adhesion.  VE-cadherin is essential for maintaining the integrity of the endothelial barrier in vivo.

Other Names: Vascular endothelial-cadherin
Structure: A 120 kD member of type II Cadherin family
Distribution: Endothelial cells
Function: Involved in endothelial cell survival, migration, contact-dependent growth inhibition, and hemophilic adhesion
Ligand Receptor: CD144
Antigen References:

1. Allport JR, et al. 2002. J. Leukocyte Biol. 71:821.
2. Hirashima M, et al. 2009. Blood 93:1253.
3. Matsuyoshi N, et al. 1997. Proc. Assoc. Am. Physicians 109:362.
4. Matsumura K, et al. 2003. Blood 101:1367.
5. Hirashima M, et al. 2009. Blood 101:2261.
6. Gotsch U, et al. 1997. J. Cell Sci. 110:583.
7. Kataoka H, et al. 1997. Dev. Growth Differ. 39:729.