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Safety Data Sheet (SDS) APC Anti-mouse CD153 Antibody     Product Data Sheet (PDF)    
APC Anti-mouse CD153 Antibody
1132045 50 µg $115.00       
Clone: RM153
Isotype: Rat IgG2b, κ
Reactivity: Mouse
Immunogen: CHO cells transfected with mouse CD153 gene (Tnfsf8)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: immunoprecipitation, immunohistochemical staining of acetone-fixed frozen sections, and in vivo blocking of CD30 binding to CD30 ligand (CD153)1-3,5.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Shimozato O, et al. 1999. Biochem. Biophys. Res. Commun. 256:519.
2. Seko Y, et al. 2001. J. Pathol. 195:593.
3. Akiba H, et al. 2000. J. Exp. Med. 191:375.
4. Zhang H and GG Meadows 2005. J. Leukocyte Biol. 78:1070.
5. Zeiser R, et al. 2007. Blood 109:2225.PubMed
6. Sun X, et al. 2010. J. Immunol. 185:2222. PubMed

PMA+ionomycin-stimulated (2 day) C57BL/6 mouse

PMA+ionomycin-stimulated (2 day) C57BL/6 mouse splenocytes were stained with CD153 (clone RM153) APC (filled histogram) or rat IgG2b, κ APC isotype control (open histogram).



Description:

CD153 is a 39-40 kD type II membrane glycoprotein, also known as CD30 ligand or CD30L. This TNF superfamily member is expressed on activated T cells. CD153 has been reported to stimulate B-cell proliferation, antigen-specific antibody production, polyclonal immunoglobulin secretion, and T cell activation and proliferation. The RM153 antibody inhibits binding of CD153 to CD30.

Other Names: CD30 ligand, CD30L, TNFSF8
Structure: TNF superfamily, 39-40 kD
Distribution: Activated T cells
Function: T-B cell costimulation
Ligand Receptor: CD30
Antigen References:

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Horie R, et al. 1998. Semin. Immunol. 106:457.
3. Shimozato O, et al. 1999. Biochem. Biophys. Res. Commun. 256:519.
4. Shanebeck KD, et al. 1995. Eur. J. Immunol. 25:2147.
5. Bowen MA, et al. 1996. J. Immunol. 156:442.