Sony Biotechnology Inc. Sony Biotechnology Inc.cart
About Us Products What's New Library News Calendar Contact Us Place Order
Safety Data Sheet (SDS) PE/Cy7 Anti-mouse CD144 VE-cadherin Antibody     Product Data Sheet (PDF)    
PE/Cy7 Anti-mouse CD144 (VE-cadherin) Antibody
1290075 25 µg $130.00       
1290080 100 µg $330.00       
Clone: BV13
Isotype: Rat IgG1, κ
Reactivity: Mouse
Immunogen: VE-cadherin-Ig fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Cy7 under optimal conditions. The solution is free of unconjugated PE/Cy7 and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Clone BV13 recognizes an epitope between aa 45 and 56, and has a binding affinity of 5-15 nM.5 Additional reported applications (for relevant formats) include: Western blotting1, blocking of cell interactions in vivo1, and immunofluorescence microscopy4.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Corada M, et al. 1999. P. Natl. Acad. Sci. USA 96:9815. (WB, Block)
2. Liao F, et al. 2000. Cancer Res. 60:6805. (FC)
3. Crosby CV, et al. 2005. Blood 105:2771. (FC)
4. Liao F, et al. 2002. Cancer Res. 62:2567. (IF)
5. May C, et al. 2005. Blood 105:4337. (epitope)

Mouse endothelial cells bEnd.3 were

Mouse endothelial cells bEnd.3 were stained with CD144 (clone BV13) PE/Cy7 (filled histogram) or rat IgG1 PE/Cy7 isotype control (open histogram).



Description:

CD144, also known as vascular endothelial-cadherin (VE-cadherin), is a 120 kD member of the type II Cadherin family.  It is an endothelial specific hemophilic adhesion molecule involved in endothelial cell survival, migration, contact-dependent growth inhibition, and homophilic adhesion.  VE-cadherin is essential for maintaining the integrity of the endothelial barrier in vivo.

Other Names: Vascular endothelial-cadherin
Structure: A 120 kD member of type II Cadherin family
Distribution: Endothelial cells
Function: Involved in endothelial cell survival, migration, contact-dependent growth inhibition, and hemophilic adhesion
Ligand Receptor: CD144
Antigen References:

1. Allport JR, et al. 2002. J. Leukocyte Biol. 71:821.
2. Hirashima M, et al. 2009. Blood 93:1253.
3. Matsuyoshi N, et al. 1997. Proc. Assoc. Am. Physicians 109:362.
4. Matsumura K, et al. 2003. Blood 101:1367.
5. Hirashima M, et al. 2009. Blood 101:2261.
6. Gotsch U, et al. 1997. J. Cell Sci. 110:583.
7. Kataoka H, et al. 1997. Dev. Growth Differ. 39:729.


This product or portions thereof is manufactured under license from GE Healthcare under U.S. Patent Numbers 5,268,486; 5,569,587; 5,627,027 and patents or pending applications that are continuations, continuations-in-part, re-examinations, divisionals, reissues or foreign equivalents thereof. THIS MATERIAL IS SUBJECT TO PROPRIETARY RIGHTS OF GE HEALTHCARE BIO-SCIENCES CORP. AND CARNEGIE MELLON UNIVERSITY AND MADE AND SOLD UNDER LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. THIS PRODUCT IS LICENSED FOR SALE ONLY FOR RESEARCH. IT IS NOT LICENSED FOR ANY OTHER USE. THERE IS NO IMPLIED LICENSE HEREUNDER FOR ANY COMMERCIAL USE.
COMMERCIAL USE shall include:

  • sale, lease, license or other transfer of the material or any material derived or produced from it;
  • sale, lease, license or other grant of rights to use this Material or any material derived or produced from it;
  • use of this material to perform services for a fee for third parties.

IF YOU REQUIRE A COMMERCIAL LICENSE TO USE THIS MATERIAL AND DO NOT HAVE ONE RETURN THIS MATERIAL, UNOPENED TO Sony Biotechnology Inc., 2100 South Oak Street, Champaign, IL 61820, USA AND ANY MONEY PAID FOR THE MATERIAL WILL BE REFUNDED.