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Safety Data Sheet (SDS) PerCP/Cy55 Anti-mouse CD11a Antibody     Product Data Sheet (PDF)    
PerCP/Cy5.5 Anti-mouse CD11a Antibody
1105615 25 µg $115.00       
1105620 100 µg $240.00       
Clone: M17/4
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: C57BL/6 mouse splenic secondary cytotoxic T cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cy5.5 under optimal conditions. The solution is free of unconjugated PerCP/Cy5.5 and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

The M17/4 antibody can block CD11a-mediated cellular adhesion. Additional reported applications of this antibody (for the relevant formats) include: immunoprecipitation1,2, in vitro blocking of cell-cell adhesion1,2 and FOXP3 expression5, and immunohistochemical staining of acetone-fixed frozen sections3. The M17/4 antibody does not block the binding of 2D7 antibody to CD11a.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cy5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application References:

1. Sanchez-Madrid F, et al. 1982. Cell Immunol. 73:1. (IP, Block)
2. Kuhlman P, et al. 1991. J. Immunol. 146:1773. (IP, Block)
3. Mizgerd JP, et al. 1997. J. Exp. Med. 186:1357. (IHC)
4. Hailman E and Allen PM. 2005. J. Immunol. 175:4847. (FC)
5. Verhagen J and Wraith DC. 2014. J. Immunol. Methods. S0022. (Block) PubMed

C57BL/6 mouse splenocytes were stained

C57BL/6 mouse splenocytes were stained with CD11a (clone M17/4) PerCP/Cy5.5 (filled histogram) or rat IgG2a, κ PerCP/Cy5.5 isotype control (open histogram).



Description:

CD11a is a 180 kD glycoprotein, also known as αL integrin, LFA-1 α, Ly-15, or Ly-21. It is a member of the integrin family, primarily expressed on lymphocytes, monocytes/macrophages, and granulocytes. In association with CD18, the CD11a/CD18 complex forms LFA-1. CD11a plays an important role in intercellular adhesion and costimulation by binding its ligands, ICAM-1 (CD54), ICAM-2 (CD102), and ICAM-3 (CD50).

Other Names: αL integrin, LFA-1 α subunit, Ly-15, Ly-21, ITGAL
Structure: Integrin family, associates with integrin β2 (CD18), 180 kD
Distribution: Lymphocytes, granulocytes, monocytes, macrophages
Function: Cellular adhesion
Ligand Receptor: ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50)
Antigen References:

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Springer TA. 1994. Cell 76:301.
3. Lub M, et al. 1995. Immunol. Today 16:479.


This product or portions thereof is manufactured under license from GE Healthcare under U.S. Patent Numbers 5,268,486; 5,569,587; 5,627,027 and patents or pending applications that are continuations, continuations-in-part, re-examinations, divisionals, reissues or foreign equivalents thereof. THIS MATERIAL IS SUBJECT TO PROPRIETARY RIGHTS OF GE HEALTHCARE BIO-SCIENCES CORP. AND CARNEGIE MELLON UNIVERSITY AND MADE AND SOLD UNDER LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. THIS PRODUCT IS LICENSED FOR SALE ONLY FOR RESEARCH. IT IS NOT LICENSED FOR ANY OTHER USE. THERE IS NO IMPLIED LICENSE HEREUNDER FOR ANY COMMERCIAL USE.
COMMERCIAL USE shall include:

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