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Safety Data Sheet (SDS) PE/Cy7 Anti-mouse CD68 Antibody     Product Data Sheet (PDF)    
PE/Cy7 Anti-mouse CD68 Antibody
1285075 25 µg $155.00       
1285080 100 µg $320.00       
Clone: FA-11
Isotype: Rat IgG2a
Reactivity: Mouse
Immunogen: Purified Con A receptor glycoproteins from the P815 cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Cy7 under optimal conditions. The solution is free of unconjugated PE/Cy7 and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

ICFC - Quality tested
FC - Validated

Application Notes:

Additional reported (for relevant formats) applications include: immunoprecipitation1, 2, Western Blot1, 2, and immunohistochemical staining of frozen sections2 and paraformaldehyde-fixed paraffin-embedded sections3.

Recommended Usage:

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Silva RP, et al. 1999. Biochem. J. 338:687. (IP, WB)
2. Rabinowitz SS, et al. 1991. J. Exp. Med. 174:827. (IP, WB, IHC)
3. Wu J, et al. 2008. P. Natl. Acad. Sci. USA 105:16934. (IHC)
4. Kayama H, et al. 2012. PNAS. 109:5010. PubMed.
5. Park S, et al. 2013. Biomaterials. 34:598. PubMed.

Thioglycolate-elicited BALB/c macrophages were fixed,

Thioglycolate-elicited BALB/c macrophages were fixed, permeabilized, and then stained with CD68 (clone FA-11) PE/Cy7 (filled histogram) or rat IgG2a, κ PE/Cy7 isotype control (open histogram).



Description:

Mouse CD68, also known as macrosialin, is an 85-115 kD member of the lysosomal-associated membrane protein (LAMP) family. It is a heavily glycosylated and predominantly intracellular protein, mainly in late endosomes. Macrosialin is the murine homolog to the human macrophage glycoprotein CD68. It is expressed on tissue macrophages, Langerhans cells and at low levels on dendritic cells. Lamp proteins may have functions relating to cell-cell interaction or cell-ligand interaction. The biological function of CD68 is not completely understood.

Other Names: Macrosialin
Structure: A member of the lysosomal-associated membrane protein (lamp) family.
Distribution: Expressed on tissue macrophages, Langerhans cells, and at low levels on dendritic cells.
Function: Involved in cell-cell interaction or cell-ligand interaction, still not completely understood.
Antigen References:

1. Ramprasad MP, et al. 1996. Proc. Natl. Acad. Sci. USA 93:14833.
2. Smith MJ, et al. 1987. J. Cell. Sci. 87:113.


This product or portions thereof is manufactured under license from GE Healthcare under U.S. Patent Numbers 5,268,486; 5,569,587; 5,627,027 and patents or pending applications that are continuations, continuations-in-part, re-examinations, divisionals, reissues or foreign equivalents thereof. THIS MATERIAL IS SUBJECT TO PROPRIETARY RIGHTS OF GE HEALTHCARE BIO-SCIENCES CORP. AND CARNEGIE MELLON UNIVERSITY AND MADE AND SOLD UNDER LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. THIS PRODUCT IS LICENSED FOR SALE ONLY FOR RESEARCH. IT IS NOT LICENSED FOR ANY OTHER USE. THERE IS NO IMPLIED LICENSE HEREUNDER FOR ANY COMMERCIAL USE.
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