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Safety Data Sheet (SDS) Brilliant Violet 421 Anti-mouse CD68 Antibody     Product Data Sheet (PDF)    
Brilliant Violet 421™ Anti-mouse CD68 Antibody
1285085 50 µg $255.00       
Clone: FA-11
Isotype: Rat IgG2a
Reactivity: Mouse
Immunogen: Purified Con A receptor glycoproteins from the P815 cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions. The solution is free of unconjugated Brilliant Violet 421™ and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

ICFC - Quality tested
FC - Validated

Application Notes:

Additional reported (for relevant formats) applications include: immunoprecipitation1, 2, Western Blot1, 2, and immunohistochemical staining of frozen sections2 and paraformaldehyde-fixed paraffin-embedded sections3.

Recommended Usage:

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Application References:

1. Silva RP, et al. 1999. Biochem. J. 338:687. (IP, WB)
2. Rabinowitz SS, et al. 1991. J. Exp. Med. 174:827. (IP, WB, IHC)
3. Wu J, et al. 2008. P. Natl. Acad. Sci. USA 105:16934. (IHC)
4. Kayama H, et al. 2012. PNAS. 109:5010. PubMed.
5. Park S, et al. 2013. Biomaterials. 34:598. PubMed.

Thioglycolate-elicited BALB/c macrophages were fixed,

Thioglycolate-elicited BALB/c macrophages were fixed, permeabilized, and then stained with CD68 (clone FA-11) Brilliant Violet 421™ (filled histogram) or rat IgG2a, κ Brilliant Violet 421™ isotype control (open histogram).



Description:

Mouse CD68, also known as macrosialin, is an 85-115 kD member of the lysosomal-associated membrane protein (LAMP) family. It is a heavily glycosylated and predominantly intracellular protein, mainly in late endosomes. Macrosialin is the murine homolog to the human macrophage glycoprotein CD68. It is expressed on tissue macrophages, Langerhans cells and at low levels on dendritic cells. Lamp proteins may have functions relating to cell-cell interaction or cell-ligand interaction. The biological function of CD68 is not completely understood.

Other Names: Macrosialin
Structure: A member of the lysosomal-associated membrane protein (lamp) family.
Distribution: Expressed on tissue macrophages, Langerhans cells, and at low levels on dendritic cells.
Function: Involved in cell-cell interaction or cell-ligand interaction, still not completely understood.
Antigen References:

1. Ramprasad MP, et al. 1996. Proc. Natl. Acad. Sci. USA 93:14833.
2. Smith MJ, et al. 1987. J. Cell. Sci. 87:113.