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Safety Data Sheet (SDS) APC Anti-rat CD62L Antibody     Product Data Sheet (PDF)    
APC Anti-rat CD62L Antibody
1614580 100 µg $220.00       
Clone: OX-85
Isotype: Mouse IgG1, κ
Reactivity: Rat
Immunogen: Rat purified CD62L-CD4
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Vosine C. 2002 J. Immunol. 169:2284.

Lewis rat splenocytes were stained

Lewis rat splenocytes were stained with CD3 FITC and CD62L (clone OX-85) APC (top) or mouse IgG1, κ APC isotype control (bottom).





Description:

CD62L is a 74-95 kD glycoprotein,also known as L-selectin, LECAM-1, Ly-22 and MEL-14.It is a member of the selectin family and is expressed on the majority of B and naïve T cells,a subset of memory T cells,granulocytes ,subsets of thymocytes and NK cells.CD62L is important in lymphocyte homing to high endothelial venules in peripheral lymph nodes and leukocyte “rolling” on activated endothelium. CD62L also contributes to neutrophil emigration at inflammatory sites.CD62L is rapidly shed from lymphocytes and neutrophils upon activation and the expression levels of CD62L (in conjunction with other markers) hae been used to distinguish naïve, effector and memory T cells.CD62L has been reported to interact with )-glycans presented on CD34, GlyCAM-1, and MadCAM-1.

Other Names: LECAM-1, Ly-22, LAM-1,MEL-14
Structure: 74-95 Kd glycoprotein of selectin family.
Distribution: Expressed on majority of B cell and naïve T cell and other leukocytes
Function: Homing and emigration of leukocytes
Regulation: Decreased expression upon cell activation