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Safety Data Sheet (SDS) PerCP/Cy55 Anti-mouse/rat CD81 Antibody     Product Data Sheet (PDF)    
PerCP/Cy5.5 Anti-mouse/rat CD81 Antibody
1124555 25 µg $155.00       
1124560 100 µg $320.00       
Clone: Eat-2
Isotype: Armenian Hamster IgG
Reactivity: Mouse, Rat
Immunogen: CD81+ mouse B lymphoma 38C13
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cy5.5 under optimal conditions. The solution is free of unconjugated PerCP/Cy5.5 and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

The Eat-2 antibody reacts with mouse and rat CD81. Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, Western blotting1,2, induction of homotypic adhesion of B lymphocytes1, stimulation of B cells undergo early apoptotic events1, and promotion of T cell motility2.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cy5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application References:

1. Maecker HT, et al. 2000. Hybridoma 19:15. (IP, WB, Stim)
2. Clark KL, et al. 2001. J. Immunol. 167:5115. (IP, WB, Activ)
3. Bhatnagar S and Schorey JS. 2007. J. Biol. Chem. doi:10.1074/jbc.M702277200. PubMed

C57BL/6 mouse splenocytes were stained

C57BL/6 mouse splenocytes were stained with mouse/rat CD81 (clone Eat-2) PerCP/Cy5.5 (filled histogram) or Armenian hamster IgG PerCP/Cy5.5 isotype control (open histogram).



Description:

CD81 is a 26 kD non-glycosylated member of the tetraspanin superfamily (TM4SF), also known as TAPA-1. CD81 is expressed on T and B cells, NK cells, dendritic cells, thymocytes, endothelial cells, and fibroblasts. CD81 induces B cell adhesion via the VLA-4 integrin and has been shown to play a role in early T cell development. CD81 associates with several other cell-surface proteins in a multimolecular complex, including CD19, CD21, CD20, CD37, CD53, and CD82 in B cells, and CD4, CD8 and CD82 in T cells.

Other Names: TAPA-1
Structure: TM4SF tetraspanin, 26 kD
Distribution: T cells and B cells, NK cells, dendritic cells, thymocytes, endothelial cells, fibroblasts
Function: Regulates cell growth
Antigen References:

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Levy S, et al. 1998. Annu. Rev. Immunol. 16:89.
3. Maeker HT, et al. 1997. FASEB J. 11:428.
4. Boismenu R, et al. 1996. Science 271:198.


This product or portions thereof is manufactured under license from GE Healthcare under U.S. Patent Numbers 5,268,486; 5,569,587; 5,627,027 and patents or pending applications that are continuations, continuations-in-part, re-examinations, divisionals, reissues or foreign equivalents thereof. THIS MATERIAL IS SUBJECT TO PROPRIETARY RIGHTS OF GE HEALTHCARE BIO-SCIENCES CORP. AND CARNEGIE MELLON UNIVERSITY AND MADE AND SOLD UNDER LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. THIS PRODUCT IS LICENSED FOR SALE ONLY FOR RESEARCH. IT IS NOT LICENSED FOR ANY OTHER USE. THERE IS NO IMPLIED LICENSE HEREUNDER FOR ANY COMMERCIAL USE.
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