The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
Clone B8.2C12 only binds to the BALB/c allele of Tim-3.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
1. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
Mouse CD366 (Tim-3) transfected cells were stained with anti-mouse CD366 (Tim-3, clone B8.2C12) APC (filled histogram) or rat IgG1, κ APC (open histogram).
CD366 (Tim-3) is a transmembrane protein also known as T cell immunoglobulin and mucin domain containing protein-3. Tim-3 is expressed at high levels on Th1 lymphocytes and CD11b+ macrophages. Tim-3 has also been shown to exist as a soluble protein. Cells expressing Tim-3 are present at high levels in the CNS of animals at the onset of experimental autoimmune encephalomyelitis (EAE), a disease mediated by lymphocytes secreting Th1-like cytokines. Tim-3 has been proposed to inhibit Th1-mediated immune responses and promote immunological tolerance.
T cell immunoglobulin and mucin domain containing protein 3, hepatitis virus cellular receptor 2, CD366
Transmembrane protein containing immunoglobulin domain and mucin-like domain. It can exist as a soluble form lacking mucin and transmembrane domains.
Expressed on activated Th1 lymphocytes, CD11b+ macrophages, and dendritic cells.
Play a role in regulating macrophage activation, T cell apoptosis and immune tolerance.
1. Sabatos CA, et al. 2003. Nat. Immunol. 4:1102 2. Kuchroo VK, et al. 2003. Nat. Rev. Immunol. 3:454 3. Mooney L, et al. 2002. Nature. 415:536 4. Rodriguez-Manzanet R, et al. 2009. Immunol. Rev. 229(1):259
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Sony, the Sony logo, Sony Biotechnology Inc. and logo, iCyt, the iCyt logo EC800 and SY3200 are trademarks of Sony Corporation. Alexa Fluor® and Pacific Blue™ are registered trademarks of and licensed under patents assigned to Life Technologies Corporation. Cy and CyDye are trademarks of GE Healthcare Limited. iCyt products containing Cy5, Cy5.5 or Cy7 are manufactured under license from GE Healthcare under U.S. Patent Numbers 5,569,587; 5,627,027 and patents or pending applications that are continuations, continuations-in-part, re-examinations, divisionals, reissues or foreign equivalents thereof. Brilliant Violet™, Brilliant Violet 421™, BV421™, Brilliant Violet 570™, BV570™, Brilliant Violet 605™, BV605™, Brilliant Violet 650™, and BV650™, Brilliant Violet 510™, BV510™, Brilliant Violet 711™, BV711™, Brilliant Violet 785™ and BV785™ are trademarks of Sirigen Group Ltd. PE-Dazzle 594™ is a trademark of Biolegend. All other trademarks are property of their respective owners. For Research Use Only. Not for use in diagnostic or therapeutic procedures.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.