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Safety Data Sheet (SDS) Purified Anti-mouse CD300LG Nepmucin Antibody     Product Data Sheet (PDF)    
Purified Anti-mouse CD300LG (Nepmucin) Antibody
1335510 100 µg $245.00       
Clone: ZAQ5
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: Nepmucin-Fc fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application:

FC - Quality tested
EM - Reported in the literature

Application Notes:

Additional reported applications for the relevant formats include: immunoelectron microscopy of peripheral LN HEVs1.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Umemoto E, et al. 2006. J. Exp. Med. 203:1603. (EM)
2. Jin S, et al. 2008. FEBS Lett. 582:3018.
3. Umemoto E, et al. 2011. Crit. Rev. Immunol. 31:147.
4. Umemoto E, et al. 2013. PLoS One 8:e83681.

Mouse CD300LG-transfected L cells were

Mouse CD300LG-transfected L cells were stained with purified CD300LG (clone ZAQ5, filled histogram) or purified rat IgG2a, κ isotype control (open histogram), followed by anti-rat IgG PE.



Description:

CD300LG, also known as nepmucin (a not expressed in Peyer's patches mucin), is a member of the CD300 antigen like family. It is a 50-95 kD glycoprotein with one IgV like domain and one mucin like domain and four isoforms are produced by alternative splicing. Nepmucin is expressed on high endothelial venules (HEV) from lymph nodes, but is absent on HEV from the Peyer's patches. CD300LG mediates lymphocyte adhesion through the intraction of its Ig domain with ICAM-1 and rolling on HEVs through the interaction of its mucin like domain with L-selectine. Also, it may play a role in the molecular transcytosis across the capillary endothelium.

Other Names: CMRF35-like molecule 9, CLM-9, CD300g, D11Ertd736e
Structure: Sialomucin, member of the CD300 antigen like family, 50-95 kD. 1 IgV like domain, 1 mucin like domain. Four isoforms produced by alternative splicing.
Distribution: Expressed on high endothelial venules (HEVs) from lymph nodes but absent on HEVs from Peyer's patches, some myeloid cell lines.
Function: Mediator of lymphocyte adhesion and rolling on HEVs, possible role in transcytosis across the capillary endothelium.
Ligand Receptor: L-selectin.
Antigen References:

1. Takatsu H, et al. 2006. Biochem. Biophys. Res. Commun. 348:183.
2. Umemoto E, et al. 2006. J. Exp. Med. 203:1603.
3. Umemoto E, et al. 2011. Crit. Rev. Immunol. 31:147.