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Safety Data Sheet (SDS) Purified Anti-mouse/human CD324 E-Cadherin Antibody     Product Data Sheet (PDF)    
Purified Anti-mouse/human CD324 (E-Cadherin) Antibody
1336505 25 µg $100.00       
1336510 100 µg $220.00       
Clone: DECMA-1
Isotype: Rat IgG1, κ
Reactivity: Mouse, Human, Cross-Reactivity: Dog (Canine), Swine (Pig, Porcine)
Immunogen: E-Cadherin extracellular domain
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application:

FC - Quality tested
IF - Validated
IP, WB, FA - Reported in the literature

Application Notes:

Additional reported applications (for relevant formats) include: immunoprecipitation1, Westen Blotting1, immunomicroscopy3, and biological function1,2.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Vestweber D, et al. 1985. EMBO. 4:3393. (IP, WB, FA)
2. Nakagawa M, et al. 2001. J. Cell Sci. 114:1829. (FA in canine cells)
3. Mohamet L, et al. 2010. PLoS ONE. 5:e12921. (IF)

MDCK epithelial cell line was

MDCK epithelial cell line was stained with purified CD324 (clone DECMA-1, filled histogram) or purified rat IgG1, κ isotype control (open histogram), followed by anti-rat IgG PE.

Canine kidney cell line MDCK

Canine kidney cell line MDCK was cultured in a chamber slide till confluent. The cells were fixed with 1% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for 10 minutes, and blocked with 5% FBS for 30 minutes. Then cells were intracellularly stained with purified 2.5 µg/ml of CD324 (E-Cadherin, clone DECMA-1) in blocking buffer overnight at 4°C followed by 2.5 µg/ml of DyLight™ 594 Goat anti-rat IgG (minimal x-reactivity) incubation for 1 hour at 4°C. Nuclei were counterstained with DAPI and are shown in blue. The image was captured with 40X objective.



Description:

CD324, also known as E-cadherin, cadherin-1, CDH1, and UVO is a member of the cadherin superfamily. It is a calcium-dependent, transmembrane cell-cell adhesion glycoprotein composed of four extracellular cadherin repeats and a highly conserved cytoplasmic tail region. CD324 is widely expressed in epithelial cells in the colon, uterus, liver, keratinocytes, brain, heart, muscle, kidney, and pancreas as well as erythroid cells. CD324 functions as a cell adhesion molecule involved in development, bacterial pathogenesis, and tumor invasion. In bacterial pathogenesis, the ectodomain of CD324 mediates bacterial adhesion to mammalian cells, while the cytoplasmic domain is required for internalization. CD324 binds to the αEβ7 integrin to mediate cell adhesion and also interacts with a number of intracellular proteins including including erbin, ezrin, caspase-3, caspase-8, β-catenin, presenilin 1, and casein kinase II as well as other extracellular proteins including the EGF receptor.

Other Names: E-Cadherin, Cadherin-1, CDH1, and UVO
Structure: Member of the cadherin superfamily. Calcium-dependent, transmembrane cell-cell adhesion glycoprotein composed of four extracellular cadherin repeats and a highly conserved cytoplasmic tail region.
Distribution: Widely expressed in epithelial cells in the colon, uterus, liver, keratinocytes, brain, heart, muscle, kidney, and pancreas as well as erythroid cells.
Function: Cell adhesion molecule involved in development, bacterial pathogenesis, and tumor invasion. The ectodomain of CD324 mediates bacterial adhesion to mammalian cells, while the cytoplasmic domain is required for internalization.
Ligand Receptor: αEβ7 integrin.
Interaction: Interacts with a variety of proteins including erbin, ezrin, caspase-3, caspase-8, EGF receptor, β-catenin, presenilin 1, casein kinase II, and others.
Antigen References:

1. Overduin M, et al. 1995. Science 267:386.
2. Boggon TJ, et al. 2002. Science 296:1308.
3. Berx G, et al. 1995. EMBO J. 14:6107.
4. Perl AK, et al. 1998. Nature 392:190.