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Safety Data Sheet (SDS) PE Anti-SSRP1 Antibody     Product Data Sheet (PDF)    
PE Anti-SSRP1 Antibody
3648525 25 tests $120.00       
3648530 100 tests $280.00       
Clone: 10D1
Isotype: Mouse IgG2b, κ
Reactivity: Human, Mouse
Immunogen: Recombinant (partial) (308-524aa)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

ICFC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining using our nuclear factor staining protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Tan BC, et al. 2006. EMBO 25:3975. (ChIP)
2. Carvalho S, et al. 2013. Nucleic Acids Res. PubMed
3. Tan BC, et al. 2004. J. Biol. Chem. 279:9321. (Immunogen)
4. Lolis AA, et al. 2013. J Biol Chem. 288:7676. PubMed
4. Aida M, et al. 2013. PNAS. 110:7784. PubMed

Human peripheral blood lymphocytes were

Human peripheral blood lymphocytes were treated with Nuclear Factor Fixation and Permeabilization Buffer Set (Cat# 422601) and then stained with anti-SSRP-1 (clone 10D1) PE (filled histogram) or mouse IgG2b, κ PE isotype control (open histogram).

C57BL/6 mouse thymocytes were treated

C57BL/6 mouse thymocytes were treated with Nuclear Factor Fixation and Permeabilization Buffer Set. (Cat# 422601) and then stained with SSRP-1 (clone 10D1) PE (filled histogram) or mouse IgG2b, κ PE isotype control (open histogram).



Description:

SSRP1 is a nuclear protein and a component of the FACT complex (facilitates chromatin transcription). The FACT complex is composed of the SUPT16H (FACT140) and the SSRP1 FACTp80 proteins. This complex interacts with nucleosomes and histone H2A/H2B dimers to promote nucleosome disassembly and allow transcription elongation. This protein interacts with dsDNA. The SSRP1 protein has been shown to be modified by cisplatin and may protect DNA from repair and block DNA replication.

Other Names: Structure specific recognition protein 1 (SSRP1), Facilitates Chromatin Transcription 80 kD subunit, FACTp80, Recombination signal sequence recognition protein
Structure: SSRP family, HMG box domain, 81 kD
Distribution: Nuclear
Function: Interacts with nucleosomes and histone H2A/H2B dimers to promote nucleosome disassembly and allow transcription elongation, binds to dsDNA. modified by cisplatin, may protect DNA from repair and block DNA replication
Interaction: Component of FACT ATP-dependent chromatin remodeling complex, SUPT16H/Cdc68 in FACT complex, H2A/H2B dimers
Antigen References:

1. LeRoy G, et al. 1998. Science 282:1836.
2. Orphanides G, et al. 1998. Cell 92:105.
3. Orphanides G, et al. 1999. Nature 400:284.