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Safety Data Sheet (SDS) Alexa Fluor 488 Anti-GATA3 Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 488 Anti-GATA3 Antibody
3869035 25 tests $120.00       
3869040 100 tests $310.00       
Clone: 16E10A23
Isotype: Mouse IgG2b, κ
Reactivity: Human, Mouse
Immunogen: Partial human GATA3 recombinant protein (1-258 aa).
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

ICFC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining using our nuclear factor staining protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Application References:

1. Pannu J, et al. 2015. PLoS One. 10:117304. PubMed

Human T leukemia cell line

Human T leukemia cell line Jurkat (filled histogram) or Burkitt's lymphoma cell line Ramos (open histogram) was treated with Nuclear Factor Fixation and Permeabilization Buffer Set , and then stained with anti-human GATA3 (clone 16E10A23) Alexa Fluor® 488.



Description:

GATA3 functions as a major regulator of T helper 2 (Th2) cell differentiation in immune cells. GATA3 expression is upregulated through IL-4 receptor signaling or the Notch-mediated pathway, which results in production of IL-4, IL-5, and IL-13 cytokines, responsible for preferential differentiation of Th2-cells. GATA3 has been shown to regulate early developmental processes of T cells, such as T cell commitment, positive selection, and post-commitment CD4+ T cell survival. In the mammary gland, GATA3 plays an important role in differentiation and commitment of luminal epithelial cells. In a mouse model, exogenous expression of GATA3 in undifferentiated breast carcinoma cells induces differentiation and suppresses spreading of the tumor cells, suggesting that GATA3 is involved in preventing malignancy of breast cancer. GATA3 has been reported to be essential in the development of a variety of normal tissues. Defect in GATA3 results in hypoparathyroidism, sensorineural deafness, and renal dysplasia (HDR syndrome).

Other Names: GATA-binding factor 3, trans-acting T-cell-specific transcription factor GATA-3
Structure: 48 kD protein containing two GATA-type zinc finger domains responsible for DNA binding and two trans-activating domains at N-terminus.
Distribution: Nucleus
Function: GATA3, a transcriptional activator, binds to a consensus DNA sequence 5'-(A/T)GATA(A/G)-3' in the promoter region of genes, regulates T cell development, TH2 cell differentiation, and luminal cell proliferation.
Interaction: Interacts with Smad3, FOG1, FOG2, and PU.1.
Antigen References:

1. Yagi R, et al. 2011. Int. Immunol. 23:415.
2. Chou J, et al. 2010. J. Cell Physiol. 222:42.
3. Ho IC, et al. 2009. Nat. Rev. Immunol. 9:125.
4. Rothenberg EV, et al. 2008. Semin. Immunol. 20:236.
5. Kouros-Mehr H, et al. 2008. Curr. Opin. Cell Biol. 20:164.
6. Van Esch H, et al. 2000. Nature 406:419.


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