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Safety Data Sheet (SDS) PerCP Anti-mouse IgD Antibody     Product Data Sheet (PDF)    
PerCP Anti-mouse IgD Antibody
2628675 25 µg $100.00       
2628680 100 µg $255.00       
Clone: 11-26c.2a
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP under optimal conditions. The solution is free of unconjugated PerCP and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

The 11-26c.2a antibody reacts with immunoglobulin D in all tested mouse haplotypes. The antibody binds membrane IgD expressed on most B cells. The 11-26c.2a antibody neither induces proliferation of splenic B cells nor induces B cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections2,3.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP has a maximum absorption of 482 nm and a maximum emission of 675 nm.

Application References:

1. Nitschke L, et al. 1993. P. Natl. Acad. Sci. USA 90:1887. (FC)
2. Weih D, et al. 2001. J. Immunol. 167:1909. (IHC)
3. Koni PA, et al. 2001. J. Exp. Med. 193:741. (IHC)
4. Ahuja A, et al. 2007. J. Immunol. 179:3351. (FC) PubMed
5. Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
6. Good-Jacobson KL, et al. 2010. Nat. Immunol. 11:535. (FC) PubMed
7. Tomayko MM, et al. 2010. J. Immunol. 185:7146. PubMed
8. Park SY, et al. 2013. J. Immunol. 190:1094. PubMed

C57BL/6 mouse splenocytes were stained

C57BL/6 mouse splenocytes were stained with CD45R/B220 APC and IgD (clone 11-26c.2a) PerCP (top) or rat IgG2a, κ PerCP isotype control (bottom).





Description:

Surface IgD is an important B cell differentiation marker.

Other Names:
Structure: Ig family
Distribution: B cells
Function: B cell differentiation