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Safety Data Sheet (SDS) PE Anti-mouse GM-CSF Antibody     Product Data Sheet (PDF)    
PE Anti-mouse GM-CSF Antibody
3127025 25 µg $75.00       
3127030 100 µg $195.00       
Clone: MP1-22E9
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: Yeast-expressed, recombinant mouse GM-CSF
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

ICFC - Quality tested

Application Notes:

ELISA or ELISPOT Capture1,3-5: The purified MP1-22E9 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MP1-31G6 antibody as the detecting antibody.

Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References:

1. Sander B, et al. 1993. J. Immunol. Methods 166:201.
2. Suda T, et al. 1990. Cell. Immunol. 129:228.
3. Nozaki S, et al. 1991. J. Invest. Dermatol. 97:10.
4. Abrams JS, et al. 1992. Immunol. Rev. 127:5.
5. Abrams JS. 2001. Curr. Protoc. Immunol. Unit 6.20.
6. Sander B, et al. 1991. Immunol. Rev. 119:65.
7. Andersson U, et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag.
8. Larkin J, et al. 2006. J. Immunol. 177:268.

PMA+ionomycin-stimulated BALB/c mouse T cells

PMA+ionomycin-stimulated BALB/c mouse T cells surface were stained with CD4 PE/Cy7 and then intracellular stained with MP1-22E9 PE



Description:

GM-CSF is a hematopoietic factor that is produced by T cells, macrophages, fibroblasts and endothelial cells. This multifunctional cytokine stimulates progenitor cells of neutrophils, eosinophils, and macrophages. GM-CSF is also a differentiation and activating factor for granulocytic and monocytic cells.

Other Names: Granulocyte/macrophage-colony stimulating factor, CSF-α, Pluripoietin-α, Eosinophil colony stimulating factor (Eo-CSF), Burst promoting activity (BPA)
Structure: Cytokine; 22 kD (Mammalian)
Regulation: Synergistic with IL-1, IL-3, G-CSF; E21R competitive antagonist for receptor binding; stored in ECM with heparan sulfate proteoglycans
Cellular Sources: T cells, monocytes/macrophages, fibroblasts, endothelial cells, mast cells
Cellular Targets: Granulocyte/macrophage/erythroid/megakaryocytic progenitors, myeloblasts, monoblasts
Receptors: Heterodimer GM-CSFR α subunit (CDw116); β-subunit (CDw131) in common with IL-3R, IL-5R
Bioactivity/Activities: Growth/development granulocyte/macrophage progenitors; differentiates myeloblasts/monoblasts; synergizes with Epo proliferation of erythroid/megakaryocytic progenitors
Antigen References:

1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Demetri, G., et al. 1991. Blood 78:2791.
3. Fan, D., et al. 1991. In vivo 5:571.
4. Negrin, R., et al. 1992. Adv. Pharmacol. 23:263.