The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
ICFC - Quality tested
ELISA or ELISPOT Capture1,3-5: The purified MP1-22E9 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MP1-31G6 antibody as the detecting antibody.
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
1. Sander B, et al. 1993. J. Immunol. Methods 166:201. 2. Suda T, et al. 1990. Cell. Immunol. 129:228. 3. Nozaki S, et al. 1991. J. Invest. Dermatol. 97:10. 4. Abrams JS, et al. 1992. Immunol. Rev. 127:5. 5. Abrams JS. 2001. Curr. Protoc. Immunol. Unit 6.20. 6. Sander B, et al. 1991. Immunol. Rev. 119:65. 7. Andersson U, et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag. 8. Larkin J, et al. 2006. J. Immunol. 177:268.
PMA+ionomycin-stimulated BALB/c mouse T cells surface were stained with CD4 PE/Cy7 and then intracellular stained with MP1-22E9 PE
GM-CSF is a hematopoietic factor that is produced by T cells, macrophages, fibroblasts and endothelial cells. This multifunctional cytokine stimulates progenitor cells of neutrophils, eosinophils, and macrophages. GM-CSF is also a differentiation and activating factor for granulocytic and monocytic cells.
Heterodimer GM-CSFR α subunit (CDw116); β-subunit (CDw131) in common with IL-3R, IL-5R
Growth/development granulocyte/macrophage progenitors; differentiates myeloblasts/monoblasts; synergizes with Epo proliferation of erythroid/megakaryocytic progenitors
1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego. 2. Demetri, G., et al. 1991. Blood 78:2791. 3. Fan, D., et al. 1991. In vivo 5:571. 4. Negrin, R., et al. 1992. Adv. Pharmacol. 23:263.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.