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Safety Data Sheet (SDS) Alexa Fluor 488 Anti-IRF4 Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 488 Anti-IRF4 Antibody
3832025 25 µg $130.00       
3832030 100 µg $310.00       
Clone: IRF4.3E4
Isotype: Rat IgG1, κ
Reactivity: Mouse, Human
Immunogen: GST fusion protein containing C-terminal of murine IRF4
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

ICFC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining using our nuclear factor staining protocol. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Application References:

1. Zheng Y, et al. 2009. Nature 458:351
2. Yin SY, et al. 2011. Exp Cell Res. 317:2210. PubMed

PHA-stimulated (3 days) or freshly

PHA-stimulated (3 days) or freshly isolated human peripheral lymphocytes were fixed and permeabilized with Nuclear Factor Fixation and Permeabilization Buffer Set . Cells were then stained with IRF4 (clone IRF4.3E4) Alexa Fluor® 488 (open histogram with solid line: fresh cells, filled histogram: stimulated cells) or rat IgG1, κ Alexa Fluor® 488 isotype control (dash line: fresh cell, dot line: stimulated cells).



Description:

The IRF family consists of at least nine members. IRF4 and IRF8 are highly homologous to each other and also redundant in function. IRF4 is critical for Th2 and Th17 development. Together with TRF8, it plays an essential role in macrophage and dendritic cell development and function. IRF4 is also reported to be essential for pre-B cell development, receptor editing, germinal center reactor and plasma cell differentiation.

Other Names: Interferon regulatory factor 4
Structure: Molecular weight is approximately 51 kD.
Distribution: IRF4 is reported to be expressed exclusively in the immune system.
Antigen References:

1. Lu R. 2008. Trends Immunol 29:487.


This product is provided under an agreement between Life Technologies Corporation and Sony Biotechnology Inc. and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications, and corresponding non-U.S. equivalents, owned by and/or licensed to Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research and/or development conducted by the buyer (whether the buyer is an academic or for profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to (1) use of the product or its components in manufacturing, (2) use of the product or its components for therapeutic, diagnostic or prophylactic purposes, or (3) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Molecular Probes Detection Technologies, Attn: Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.