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Safety Data Sheet (SDS) PE Anti-mouse IL-3 Antibody     Product Data Sheet (PDF)    
PE Anti-mouse IL-3 Antibody
3119515 25 µg $95.00       
3119520 100 µg $235.00       
Clone: MP2-8F8
Isotype: Rat IgG1, κ
Reactivity: Mouse
Immunogen: COS-expressed, recombinant mouse IL-3
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

ICFC - Quality tested

Application Notes: ELISA or ELISPOT Capture1-5,8: The purified MP2-8F8 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MP2-43D11 antibody as the detecting antibody.
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References: 1. Abrams, J., et al. 1992. Immunol. Rev. 127:5.
2. Abrams, J., et al. 1988. J. Immunol. 140:131.
3. Cockayne, D., et al. 1991. Growth Factors 5:171.
4. Sander, B., et al. 1993. J. Immunol. Meth. 166:201.
5. Abrams, J. 1995. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.20.
6. Finkelman, F., et al. 1993. J. Immunol. 151:1235.
7. Andersson, U., et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag.
8. Karulin, A., et al. 2000. J. Immunol. 164:1862.
PMA+Ionomycin stimulated Th2-polarized C57BL/6 splenocytes

PMA+Ionomycin stimulated Th2-polarized C57BL/6 splenocytes surface stained with CD4 (GK1.5) APC and intracellularly stained with MP2-8F8 PE



Description: IL-3 is a highly species-specific pleiotropic factor produced primarily by activated T cells though also by mast cells keratinocytes, and astrocytes, which stimulates colony formation of megakaryocytes, neutrophils, and macrophages from bone marrow cultures. The MP2-8F8 antibody reacts with mouse interleukin-3 (IL-3). The MP2-8F8 antibody can neutralize the bioactivity of natural or recombinant IL-3.
Other Names: Interleukin-3, Burst promoting activity (BP/BPA), Eosinophil colony stimulating factor (Eo-CSF), Hematopoietic cell growth factor (HCGF), Mast cell growth factor (MGF/MCGF), Multi-colony stimulating factor (Multi-CSF), P cell stimulating activity (PCSA),
Structure: Cytokine; 28 kD (Mammalian)
Regulation: Upregulated by T cell stimulation; downregulated by glucocorticoids
Cellular Sources: Activated T cells, mast cells, eosinophils, keratinocytes, NK cells, endothelial cells
Cellular Targets: Erythroid cells, megakaryocytes, neutrophils, eosinophils, basophils, mast cells, monocytic lineages
Receptors: Heterodimer IL-3Rα (CD123) and two alternate β-subunits, AIC2A binds IL-3 and is specific to IL-3Rα, AIC2B (CDw131) shared in common with IL-5R, GM-CSFR
Bioactivity/Activities: Proliferation/differentiation of almost all types of hematopoietic progenitors into granulocytes, macrophages, erythroid cells, megakaryocytes, mast cell colonies; induces expression of 20-α-steroid dehydrogenase, histidine, ornithine decarboxylase
Antigen References: 1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Frendl, G., 1992. Int. J. Immunopharmacol. 14:421.
3. Ihle, J., 1992. Chem. Immunology 51:65.
4. Valen T, P., et al. 1990. Blut 61:338.