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Safety Data Sheet (SDS) Purified Anti-mouse GITR Ligand Antibody     Product Data Sheet (PDF)    
Purified Anti-mouse GITR Ligand Antibody
1337010 100 µg $220.00       
Clone: 5F1
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application:

FC - Quality tested

Application Notes:

Additonal reported applications (for relevant formats of thes clone) include: inhibition of GITR-GITRL mediated T cell proliferation and reversing Treg-mediated suppression1.

The following patents apply to clone 5F1: U.S. Patent Serial No. 7,618,632 and Japan Patent Serial No. 4638876.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Stephens GL, et al. 2004. J. Immunol. 173:5008. (Block)

GITR ligand transfected cells were

GITR ligand transfected cells were stained with purified GITR ligand (clone 5F1, filled histogram) or purified rat IgG2a, κ isotope control (open histogram), followed by anti-rat IgG PE.



Description:

GITR ligand (GITRL) is a member of the TNF superfamily, also known as TNFSF18 or TL6. It is expressed on B cells, macrophages, dendritic cells, and endothelial cells. The expression of GITRL on the dendritic cell surface can be transiently upregulated, and then rapidly downregulated, by LPS stimulation. GITRL binds to its receptor, GITR, to costimulate T cell activation, proliferation, and cytokine production, inhibit the suppressive functions of CD4+CD25+ Tregs, and induce macrophage activation. GITR-GITRL interaction has been implicated as an essential regulator of the immune response to self antigens and phathogens.

Other Names: GITRL, AITRL, TNFSF18, TL6
Structure: TNF superfamily member
Distribution: B cells, macrophages, dendritic cells, endothelial cells
Function: Binds GITR receptor to costimulate T cell activation, proliferation, and cytokine production; inhibits the suppressive activities of CD4+CD25+ Tregs
Antigen References:

1. Tone M, et al. 2003. Proc. Natl. Acad. Sci. USA 100:15059.
2. Kim JD, et al. 2003. Genes Immun. 4:564.
3. Yu KY, et al. 2003. Biochem. Biophys. Res. Commun. 310:433.
4. Shin HH, et al. 2002. Cytokine 19:187.