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Safety Data Sheet (SDS) PerCP/Cy55 Anti-mouse CD2 Antibody     Product Data Sheet (PDF)    
PerCP/Cy5.5 Anti-mouse CD2 Antibody
1100575 25 µg $100.00       
1100580 100 µg $270.00       
Clone: RM2-5
Isotype: Rat IgG2b, λ
Reactivity: Mouse
Immunogen: BALB/c mouse thymocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cy5.5 under optimal conditions. The solution is free of unconjugated PerCP/Cy5.5 and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: in vitro blocking of CD2-mediated cell-cell adhesion and inhibition of T cell activation1, blocking of T cell A.I.C.D.2, immunoprecipitation3, and co-induction of thymocyte maturation4. The RM2-5 antibody can block CD2-mediated cell-cell adhesion.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cy5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application References:

1. Nakamura T, et al. 1990. J. Immunol. 145:3628. (Block)
2. Ayroldi E, et al. 1997. Blood 89:3717. (Block)
3. Criado G, et al. 1996. Eur. J. Immunol. 26:1228. (IP)
4. Cibotti R, et al. 1997. Immunity 6:245. (Costim)

C57BL/6 mouse splenocytes were stained

C57BL/6 mouse splenocytes were stained with CD2 (clone RM2-5) PerCP/Cy5.5 (filled histogram) or rat IgG2b, κ PerCP/Cy5.5 isotype control (open histogram).



Description:

CD2 is a 45-58 kD type I transmembrane glycoprotein, also known as LFA-2, T11 or Ly-37. It is a member of the Ig superfamily. Mouse CD2 is primarily expressed on T cells, B cells, thymocytes and NK cells. It is a ligand for CD48 and is involved in T cell activation and differentiation.

Other Names: LFA-2, T11, Ly-37, SRBC-R
Structure: Ig superfamily, 45-58 kD
Distribution: T cells, thymocytes, NK cells, B cells
Function: T cell activation, adhesion
Ligand Receptor: CD48, lck
Antigen References:

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis SJ, et al. 1996. Immunol. Today 17:177.
3. Bierer BE, et al. 1989. Annu. Rev. Immunol. 7:579.


This product or portions thereof is manufactured under license from GE Healthcare under U.S. Patent Numbers 5,268,486; 5,569,587; 5,627,027 and patents or pending applications that are continuations, continuations-in-part, re-examinations, divisionals, reissues or foreign equivalents thereof. THIS MATERIAL IS SUBJECT TO PROPRIETARY RIGHTS OF GE HEALTHCARE BIO-SCIENCES CORP. AND CARNEGIE MELLON UNIVERSITY AND MADE AND SOLD UNDER LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. THIS PRODUCT IS LICENSED FOR SALE ONLY FOR RESEARCH. IT IS NOT LICENSED FOR ANY OTHER USE. THERE IS NO IMPLIED LICENSE HEREUNDER FOR ANY COMMERCIAL USE.
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