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Safety Data Sheet (SDS) FITC Anti-mouse Qa-2 Antibody     Product Data Sheet (PDF)    
FITC Anti-mouse Qa-2 Antibody
1208545 50 µg $75.00       
1208550 500 µg $275.00       
Clone: 695H1-9-9
Isotype: Mouse IgG2a, κ
Reactivity: Mouse
Immunogen: C3H.SW mouse skin graft and splenocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC and unconjugated antibody.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: immunoprecipitation2.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Sharrow SO, et al. 1989. J. Immunol. 142:3495.
2. Patk E, et al. 1993. Microbiol. Immunol. 37:743.

C57BL/6 mouse splenocytes were stained

C57BL/6 mouse splenocytes were stained with Qa-2 (clone 695H1-9-9) FITC (filled histogram) or mouse IgG2a FITC isotype control (open histogram).



Description:

Mouse Qa-2 is a 40 kD GPI-linked surface protein. It is a member of nonclassical MHC class Ib molecule encoded by Q5, Q6, Q7, Q9, and Q10 genes of the H-2 complex. Qa-2 is a nonpolymorphic molecule primarily expressed on mouse T and B cells. The expression level of Qa-2 on mature T cells is higher than on B cells. Different mouse strains express Qa-2 at varying levels. Qa-2, in association with β2-microglobulin, binds to peptide and plays a role in antigen presentation.

Other Names: Nonclassical MHC-Ib, H2-Qa2
Structure: GPI-linked protein, associates with β2-microglobulin, 40 kD
Distribution: T and B cells, mature T cells expression higher than B cells
Function: Antigen presentation, promote immune response
Ligand Receptor: Peptide
Antigen References:

1. Sharrow SO, et al. 1989. J. Immunol. 142:3495.
2. Mann DW, et al. 1987. J. Immunol. 138:240.
3. Mellor AL, et al.1985. Proc. Natl. Acad. Sci.USA. 82:5920.
4. Tabaczewski P, et al. 1997. J. Immunol. 159:2771.
5. Chiang EY and Stroynowski I. 2005. J. Immunol. 174:5367.