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Safety Data Sheet (SDS) Brilliant Violet 510 Annexin V     Product Data Sheet (PDF)    
Brilliant Violet 510™ Annexin V
3804685 25 tests $160.00       
Reactivity: All mammalian species
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The protein was purified by affinity chromatography and conjugated with Brilliant Violet 510™ under optimal conditions.
Concentration: Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling: The solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Annexin V Staining 
1.
2. Transfer 100 µl of cell suspension in 5 ml test tube.
3. Add 5 µl of Brilliant Violet 510™ Annexin V.
4. Add 10 µl of PI solution or 7-AAD . 
5. Gently vortex the cells, and incubate for 15 min at room temperature (25°C), in the dark. 
6. Add 400 µl of Annexin V Binding Buffer to each tube. Analyze by flow cytometry.

Recommended Usage:

Each lot of this product is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per 100,000 - million cells in a 100 µl volume of Annexin V Binding Buffer (Cat No. 422201). It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 510™ excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510™ is a trademark of Sirigen Group Ltd.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Application References:

1. Koopman G, et al. 1994. Blood 84:1415. 
2. Vermes I, et al. 1995. J. Immunol. Methods 184:39. 
3. Dachary-Prigent J, et al. 1993. Blood 81:2554.
4. Sekine C, et al. 2009. Int Immunol. 25:295. PubMed
5. Grujic M, et al. 2010. J. Immunol. 185:1730. PubMed

Human T-cell leukemia cell line,

Human T-cell leukemia cell line, Jurkat, was treated with (filled histogram) or without (open histogram) LEAF™ purified anti-CD95 (clone EOS9.1) for 4 hours, then stained with Annexin V Brilliant Violet 510™.



Description:

Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner. PS is normally only found on the intracellular leaflet of the plasma membrane in healthy cells, but during early apoptosis, membrane asymmetry is lost and PS translocates to the external leaflet. Fluorochrome-labeled Annexin V can then be used to specifically target and identify apoptotic cells. Annexin V Binding Buffer is recommended for use with Annexin V staining. Annexin V binding alone cannot differentiate between apoptotic cells and necrotic. So, we recommend using our 7-AAD Viability Staining Solution or Propidium Iodide Solution . Early apoptotic cells will exclude 7-AAD and PI, while late stage apoptotic cells and necrotic cells will stain positively, due to the passage of these dyes into the nucleus where they bind to DNA.

Other Names: Annexin A5