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Safety Data Sheet (SDS) PE Anti-mouse CD104 Antibody     Product Data Sheet (PDF)    
PE Anti-mouse CD104 Antibody
1218045 25 µg $120.00       
1218050 100 µg $280.00       
Clone: 346-11A
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: Tumor-associated antigen TSP-180 from BALB/c lung carcinoma
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported (for the relevant formats) applications include: immunohistochemical staining1.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Tennenbaum T, et al. 1993. Cancer Res. 53:4803. (IHC)

Mouse lung carcinoma cell line

Mouse lung carcinoma cell line (Line 1) was stained with CD104 (clone 346-11A) PE (filled histogram) or rat IgG2a, κ PE isotype control (open histogram).



Description:

CD104 is a 205 kD type I transmembrane glycoprotein, also known as integrin β4 chain or β4 integrin, that associates with integrin α6 (CD49f) to form the α6/β4 (CD49f/CD104) heterodimer. CD104 is expressed on epithelial cells (especially on proliferative basal layer epithelial cells in skin), endothelial cells, Schwann cells, certain tumor cells, and a subset of pre-T cells. The CD49f/CD104 heterodimer is an adhesion receptor for laminins (especially laminin 5) and keratin filaments, and is involved in the regulation of hemidesmosome formation and cell proliferation and activation.

Other Names: Integrin β4, β4 integrin, TSP-1180, ITGB4
Structure: Integrin β chain, 205 kD, forms heterodimer with integrin α6 (CD49f)
Distribution: Epithelial cells, endothelial cells, some tumor cells, pre-T cell subset
Function: Adhesion
Ligand Receptor: Laminins, keratin filaments
Antigen References:

1. Kennel SJ, et al. 1992. J. Cell Sciences 101:1992.