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Safety Data Sheet (SDS) PE/Dazzle 594 Anti-mouse CD11c Antibody     Product Data Sheet (PDF)    
PE/Dazzle™ 594 Anti-mouse CD11c Antibody
1186735 25 µg $105.00       
1186740 100 µg $275.00       
Clone: N418
Isotype: Armenian Hamster IgG
Reactivity: Mouse
Immunogen: Mouse spleen dendritic cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions. The solution is free of unconjugated PE/Dazzle™ 594 and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: immunoprecipitation3, immunohistochemical staining of acetone-fixed frozen sections3, and immunofluorescence microscopy5, 9 (Alexa Fluor® 488 conjugated N418 was used for IHC in frozen sections10).

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.

Application References:

1. Granucci F, et al. 1997. J. Immunol. 159:1794.
2. Stokes RW, et al. 1998. J. Immunol. 160:5514.
3. Metlay JP, et al. 1990. J. Exp. Med. 171:1753. (IHC, IP)
4. Ma XT, et al. 2006. Cancer Research 66:1169.
5. Chin RK, et al. 2006. J. Immunol. 177:290. (IF)
6. Cervantes-Barragan L, et al. 2007. Blood 109:1131. (FC) PubMed
7. Turnquist HR, et al. 2007. J. Immunol. 178:7018. (FC) PubMed
8. Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719. (FC) PubMed
9. You Y, et al. 2009. J. Immunol. 182:7343. (IF) PubMed
10. Roland CL, et al. 2009. Mol. Cancer Res. 8:1761. (IHC, FC) PubMed
11. Wikstrom M, et al.2006. J. Immunol. 177:913. PubMed
12. Pericolini E, et al. 2008. J. Leukocyte Biol. 83:1286. PubMed
13. Randall LM, et al. 2008. Infect. Immun.76:3312. PubMed
14. Fahlen-Yrild L, et al. 2009. J. Immunol. 183:5032. PubMed
15. Osterholzer JJ, et al. 2009. J. Immunol. 183:8044. PubMed
16. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
17. Eisenach PA, et al. 2010. J Cell Sci. 123:4182. PubMed
18. Leppin K, et al. 2014. Invest. Ophthalmol. Vis. Sci. 55:3603. PubMed

C57BL/6 mouse splenocytes were stained

C57BL/6 mouse splenocytes were stained with I-A/I-E FITC and CD11c (clone N418) PE/Dazzle™ 594.



Description:

CD11c is a 150 kD glycoprotein also known as αX integrin, CR4, and p150. CD11c forms a αXβ2 heterodimer with β2 integrin (CD18). It is primarily expressed on dendritic cells, NK cells, a subset of intestinal intraepithelial lymphocytes (IEL), and some activated T cells. The αXβ2 integrin plays an important role in cell-cell contact by binding its ligands: iC3b, fibrinogen, and CD54.

Other Names: αX integrin, integrin αX chain, CR4, p150, ITGAX
Structure: Integrin α-chain, associates with integrin β2 (CD18), 150 kD
Distribution: Dendritic cells, NK cells, intestinal intraepithelial lymphocytes (IEL), some activated T cells
Function: Cellular adhesion
Ligand Receptor: iC3b, fibrinogen
Antigen References:

1. Barclay A, et al. 1997. The Leukocyte Antigen Facts Book Academic Press.
2. Springer TA. 1994. Cell 76:301.
3. Lopez-Rodriguez C, et al. 1996. J. Immunol. 156:3780.