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Safety Data Sheet (SDS) Alexa Fluor 647 Anti-Tubulin Beta 3 TUBB3 Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 647 Anti-Tubulin Beta 3 (TUBB3) Antibody
3887025 25 µg $155.00       
3887030 100 µg $325.00       
Clone: AA10
Isotype: Mouse IgG2a, κ
Reactivity: Mouse, Rat, Human
Immunogen: Fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

ICFC - Quality tested
IHC-F - Validated

Recommended Usage:

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. For immunohistochemical staining on frozen tissue sections, the suggested use is 1.25-5 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Human lung adenocarcinoma cell line

Human lung adenocarcinoma cell line A549 was treated with Fixation Buffer (Cat# 420801) and Permeabilization Wash Buffer (Cat# 421002), and then stained with TUBB3 (clone AA10) Alexa Fluor® 647 (filled histogram) or mouse IgG2a, κ Alexa Fluor® 647 isotype control (open histogram).

C57BL/6 mouse frozen brain tissue

C57BL/6 mouse frozen brain tissue was fixed with 4% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5 % Triton X-100 for ten minutes, and blocked with 5% FBS for 1 hour. Then the tissue was stained with 1.25 µg/ml of Alexa Fluor® 647 anti-Tubulin Beta 3 (TUBB3) (Clone AA10) (shown in red) and 5 µg/ml of Alexa Fluor® 488 anti-GFAP (Clone 2E1.E9) (shown in green) in blocking buffer, overnight at 4°C. Nuclei were counterstained with DAPI (blue). The image was captured with 10X objective.



Description:

Tubulin is the main component of microtubules. In adults, tubulin beta 3 (TUBB3) is primarily expressed in neurons and is commonly used as a neuronal marker. It plays an important role in neuronal cell proliferation and differentiation. Mutations in this gene cause congenital fibrosis of the type 3 extraocular muscles. Tubulin beta 3 (TUBB3) is also found in a wide range of tumors. Studies indicate that it is a predictive and prognostic marker in various tumors.

Other Names: β-3 Tubulin, Neuronal class III beta-tubulin, β3-tub
Structure: 450 amino acids with predicted molecular weight of 50 kD
Distribution: Cytosol
Function: Plays important roles in neuronal cell proliferation and differentiation
Interaction: Alpha tubulin, kinesin and dynein
Antigen References:

1. Katsetos CD, et al. 2003. J. Child Neurol. 18:851.
2. Mobarakeh ZT, et al. 2012. Cell Biol. Int. Rep. (2010) 19:e00015.
3. Locher H, et al. 2013. Differentiation. 85:173.
4. Karki R, et al. 2013. Expert Opin. Ther. Targets. 17:461.
5. Mariani M, et al. 2011. Curr. Mol. Med. 11:726.
6. Koh Y, et al. 2009. Ann. Oncol. 20:1414.


This product is provided under an agreement between Life Technologies Corporation and Sony Biotechnology Inc. and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications, and corresponding non-U.S. equivalents, owned by and/or licensed to Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research and/or development conducted by the buyer (whether the buyer is an academic or for profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to (1) use of the product or its components in manufacturing, (2) use of the product or its components for therapeutic, diagnostic or prophylactic purposes, or (3) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Molecular Probes Detection Technologies, Attn: Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.