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Safety Data Sheet (SDS) Alexa Fluor 647 Anti-human/mouse Cutaneous Lymphocyte Antigen CLA Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 647 Anti-human/mouse Cutaneous Lymphocyte Antigen (CLA) Antibody
2206545 25 tests $80.00       
2206550 100 tests $220.00       
Clone: HECA-452
Isotype: Rat IgM, κ
Workshop Number: V S075
Reactivity: Human, Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

The HECA-452 antibody cross-reacts with mouse skin homing lymphocytes4. Treatment of activated HUVEC cells with HECA-452 antibody inhibits lymphocyte adhesion. Additional reported applications (for the relevant formats) include: blocking of lymphocyte binding to E-selectin3, and immunohistochemistry1,2 of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Application References:

1. Duijvestijn AM, et al. 1988. Am. J. Pathol. 130:147. (IHC)
2. Picker LJ, et al. 1991. Nature 349:796. (IHC)
3. Berg EL, et al. 1991. J. Exp. Med. 174:1461.
4. Borges E, et al. 1997. J. Biol. Chem. 272:28786.
5. Ren YL, et al. 2012. Am J Clin Pathol. 138:435. PubMed

Human peripheral blood lymphocytes were

Human peripheral blood lymphocytes were stained with CLA (clone HECA-452) Alexa Fluor® 647 (filled histogram) or rat IgM Alexa Fluor® 647 isotype control (open histogram).



Description:

Cutaneous lymphocyte antigen (CLA) is a 140 kD homodimer protein recognized by a unique mAb, HECA-452. It is expressed on T cells in skin, subsets of peripheral blood memory T cells, NK cells, memory B cells and dendritic cells as well as on monocytes, granulocytes, and activated endothelial cells. CLA is a carbohydrate epitope of sialic acid and fucose-modified P-selectin glycoprotein ligand-1 (PSGL-1), a surface glycoprotein expressed on the majority of peripheral blood leukocytes. CLA is a ligand for E-selectin, P-selectin, and L-selectin. It plays a role in memory lymphocyte homing, tethering, and rolling.

Other Names: Cutaneous Lymphocyte-associated Antigen (CLA)
Structure: 140 kD, carbohydrate epitope of PSGL-1
Distribution: T cells in skin, subsets of peripheral blood T cells, NK cells, B cells and dendritic cells, monocytes, granulocytes
Function: Memory cells tethering and rolling
Ligand Receptor: E-selectin, P-selectin, L-selectin
Antigen References:

1. Picker LJ, et al. 1990. Am. J. Pathol. 136:1053.
2. Berg EL, et al. 1991. J. Exp. Med. 174:1461.
3. Fuhlbrigge RC, et al. 1997. Nature 389:978.
4. Tu L, et al. 1999. J. Exp. Med. 189:241.
5. Yoshino T, et al. 1999. Cell. Immunol. 197:39.
6. Chang SE, et al. 2003. Acta Derm-Venereol. 83:162.
7. Schakel K, et al. 2002. Immunity 17:289.
8. Fuhlbrigge RC, et al. 2002. J. Immunol. 168:5645.


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