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Safety Data Sheet (SDS) APC/Cy7 Anti-mouse Ig Light Chain Kappa Antibody     Product Data Sheet (PDF)    
APC/Cy7 Anti-mouse Ig Light Chain κ Antibody
2647515 25 µg $100.00       
2647520 100 µg $270.00       
Clone: RMK-45
Isotype: Rat IgG
Reactivity: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with APC/Cy7 under optimal conditions. The solution is free of unconjugated APC/Cy7 and unconjugated antibody.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

The RMK-12 monoclonal antibody may be used as a primary antibody for ELISA or as secondary for detection of mouse Ig kappa.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

C57BL/6 splenocytes were stained with

C57BL/6 splenocytes were stained with anti-mouse Ig light chain κ (clone RMK-45) APC/Cy7 (filled histogram) or rat IgG1, κ APC/Cy7 isotype control (open histogram).

Human peripheral blood lymphocytes were

Human peripheral blood lymphocytes were stained with (filled histogram) or without (open histogram) purified mouse IgG1, κ anti-human CD3 (clone UCHT1) followed by anti-mouse Ig light chain κ (clone RMK-45) APC/Cy7.



Description:

The RMK-45 monoclonal antibody reacts with immunoglobulin light chain kappa in all tested mouse haplotype (Igh-a and b). It does not react with the lambda chain.

Other Names: Immunoglobulin light chain kappa
Structure: Immunoglobulin family
Antigen References:

1. Ludwig TE, et al. 2006. Nat. Methods. 3:637.
2. Nguyen DH, et al. 2005. J. Immunol. 175:228.
3. Bardor M, et al. 2005. J. Biol. Chem. 280:4228.
4. Diaz SL, et al. 2009. PLoS One. 4:e4241.


This product or portions thereof is manufactured under license from GE Healthcare under U.S. Patent Numbers 5,268,486; 5,569,587; 5,627,027 and patents or pending applications that are continuations, continuations-in-part, re-examinations, divisionals, reissues or foreign equivalents thereof. THIS MATERIAL IS SUBJECT TO PROPRIETARY RIGHTS OF GE HEALTHCARE BIO-SCIENCES CORP. AND CARNEGIE MELLON UNIVERSITY AND MADE AND SOLD UNDER LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. THIS PRODUCT IS LICENSED FOR SALE ONLY FOR RESEARCH. IT IS NOT LICENSED FOR ANY OTHER USE. THERE IS NO IMPLIED LICENSE HEREUNDER FOR ANY COMMERCIAL USE.
COMMERCIAL USE shall include:

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  • use of this material to perform services for a fee for third parties.

IF YOU REQUIRE A COMMERCIAL LICENSE TO USE THIS MATERIAL AND DO NOT HAVE ONE RETURN THIS MATERIAL, UNOPENED TO Sony Biotechnology Inc., 2100 South Oak Street, Champaign, IL 61820, USA AND ANY MONEY PAID FOR THE MATERIAL WILL BE REFUNDED.