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Safety Data Sheet (SDS) Alexa Fluor 700 Anti-mouse Ig Light Chain Kappa Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 700 Anti-mouse Ig Light Chain κ Antibody
2647535 25 µg $100.00       
2647540 100 µg $270.00       
Clone: RMK-45
Isotype: Rat IgG
Reactivity: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Application Notes:

The RMK-12 monoclonal antibody may be used as a primary antibody for ELISA or as secondary for detection of mouse Ig kappa.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

C57BL/6 splenocytes were stained with

C57BL/6 splenocytes were stained with anti-mouse Ig light chain κ (clone RMK-45) Alexa Fluor® 700 (filled histogram) or rat IgG1, κ Alexa Fluor® 700 isotype control (open histogram).

Human peripheral blood lymphocytes were

Human peripheral blood lymphocytes were stained with (filled histogram) or without (open histogram) purified mouse IgG1, κ ant-human CD3 (clone UCHT1), followed by anti-mouse Ig light chain κ (clone RMK-45) Alexa Fluor® 700.


The RMK-45 monoclonal antibody reacts with immunoglobulin light chain kappa in all tested mouse haplotype (Igh-a and b). It does not react with the lambda chain.

Other Names: Immunoglobulin light chain kappa
Structure: Immunoglobulin family
Antigen References:

1. Ludwig TE, et al. 2006. Nat. Methods. 3:637.
2. Nguyen DH, et al. 2005. J. Immunol. 175:228.
3. Bardor M, et al. 2005. J. Biol. Chem. 280:4228.
4. Diaz SL, et al. 2009. PLoS One. 4:e4241.

This product is provided under an agreement between Life Technologies Corporation and Sony Biotechnology Inc. and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications, and corresponding non-U.S. equivalents, owned by and/or licensed to Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research and/or development conducted by the buyer (whether the buyer is an academic or for profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to (1) use of the product or its components in manufacturing, (2) use of the product or its components for therapeutic, diagnostic or prophylactic purposes, or (3) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Molecular Probes Detection Technologies, Attn: Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.