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Safety Data Sheet (SDS) FITC Anti-mouse Ig Light Chain Kappa Antibody     Product Data Sheet (PDF)    
FITC Anti-mouse Ig Light Chain κ Antibody
2647545 50 µg $85.00       
2647550 500 µg $195.00       
Clone: RMK-45
Isotype: Rat IgG
Reactivity: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC and unconjugated antibody.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

The RMK-12 monoclonal antibody may be used as a primary antibody for ELISA or as secondary for detection of mouse Ig kappa.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

C57BL/6 splenocytes were stained with

C57BL/6 splenocytes were stained with anti-mouse Ig light chain κ (clone RMK-45) FITC (filled histogram) or rat IgG1, κ FITC isotype control (open histogram).

Human peripheral blood lymphocytes were

Human peripheral blood lymphocytes were stained with (filled histogram) or without (open histogram) purified mouse IgG1, κ anti-human CD3 (clone UCHT1), followed by anti-mouse Ig light chain κ (clone RMK-45) FITC.



Description:

The RMK-45 monoclonal antibody reacts with immunoglobulin light chain kappa in all tested mouse haplotype (Igh-a and b). It does not react with the lambda chain.

Other Names: Immunoglobulin light chain kappa
Structure: Immunoglobulin family
Antigen References:

1. Ludwig TE, et al. 2006. Nat. Methods. 3:637.
2. Nguyen DH, et al. 2005. J. Immunol. 175:228.
3. Bardor M, et al. 2005. J. Biol. Chem. 280:4228.
4. Diaz SL, et al. 2009. PLoS One. 4:e4241.