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Safety Data Sheet (SDS) Purified Anti-mouse CD285 TLR5 Antibody     Product Data Sheet (PDF)    
Purified Anti-mouse CD285 (TLR5) Antibody
1340510 100 µg $220.00       
Clone: ACT5
Isotype: Mouse IgG1, κ
Reactivity: Mouse
Immunogen: TLR5-transfected Ba/F3 cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application:

FC - Quality tested
IP - Reported in the literature

Application Notes:

Additional reported applications for the relevant formats include: immunoprecipitation of TLR5 from cell lysates1,2.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Shibata T, et al. Int Immunol. 24:613. (IP, FC)
2. Huh JW, et al. 2014. Proc. Natl. Acad. Sci. USA. 111:7072. (IP)

TLR5-transfected Ba/F3 cells were stained

TLR5-transfected Ba/F3 cells were stained with purified TLR5 (clone ACT5, filled histogram) or purified mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG PE.



Description:

CD285, also known as TLR5, is a 98 kD pattern recognition receptor that binds to bacterial flagellin. It is expressed on dendritic cells, classical monocytes and mucosal epithelial cells and is involved in activation of TLR5 induced NF-κB mediated activation of inflammatory-related target genes. Mutations in the TLR5 gene have been linked with susceptibility to systemic lupus erythematosus and Legionnaire’s disease.

Other Names: Toll-like receptor 5, TIL3, SLEB1, MELIOS
Structure: Type 1 transmembrane protein, 98 kD
Distribution: Neutrophils, CD11b, Ly6C, classical monocytes, dendritic cells, mucosal epithelium
Ligand Receptor: Flagellin
Interaction: Interacts with MyD88 and TRAF6 leading to NF-κB activation and production of cytokines.
Antigen References:

1. López-Yglesias AH, et al. 2014. J. Immunol. 192:1587. 
2. Burdelya L, et al. 2013. Proc. Natl. Acad. Sci. USA. 110:E1857. 
3. Carapau D, et al. 2013. Infect. Immun. 81:4350. 
4. Kinnebrew M, et al. 2012. Immunity. 36:276. 
5. Sebastiani G, et al. 2000. Genomics. 64:230.