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Safety Data Sheet (SDS) PE/Cy7 Anti-mouse CD103 Antibody     Product Data Sheet (PDF)    
PE/Cy7 Anti-mouse CD103 Antibody
1207125 25 µg $130.00       
1207130 100 µg $310.00       
Clone: 2E7
Isotype: Armenian Hamster IgG
Reactivity: Mouse
Immunogen: Mouse intestinal intraepithelial lymphocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Cy7 under optimal conditions. The solution is free of unconjugated PE/Cy7 and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemical staining1,7 of acetone-fixed frozen sections, immunofluorescence2, and in vitro activation1.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.65 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. LeFrancois L, et. al, 1994. Eur. J. Immunol. 24:635. (FC, IHC, IP)
2. Mysorekar IU, et. al, 2002. J. Biol. Chem. 277:37811. (FC, IF)
3. Mikami N, et al. 2011. J. Immunol. 186:6886. PubMed
4. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
5. Quinn KM, et al. 2013. J. Immunol. 191:5085. PubMed
6. Verhagen J and Wraith DC. 2014. J. Immunol. Methods. S0022. (FC) PubMed
7. Xiao B, et al. 2015. PLoS One 1:e0115333. (IHC)

C57BL/6 mouse splenocytes were stained

C57BL/6 mouse splenocytes were stained with CD3 APC and CD103 (clone 2E7) PE/Cy7 (top) or Armenian hamster PE/Cy7 isotype control (bottom).





Description:

CD103 is a type I transmembrane glycoprotein known as αE integrin or Integrin αIEL chain. It belongs to the integrin family and is primarily found on intestinal intraepithelial lymphocytes (IEL). CD103 is also expressed on a subpopulation of lamina propria T cells, epithelial dendritic cells, lamina propria-derived dendritic cells, and a small subset of peripheral lymphocytes. T regulatory cells express high level of CD103. The CD103 expression on lymphocytes can be induced upon activation and TGF-β stimulation. In association with integrin β7, CD103 is expressed as αE/β7 heterodimer. Mature CD103 protein can be cleaved into 2 chains, a 150 kD (C-terminal) chain and a 25 kD (N-terminal) chain, which remain linked by disulfide bonds. CD103 binds to E-cadherin and mediates homing of lymphocytes to the intestinal epithelium.

Other Names: Integrin αIEL chain, Integrin αE chain, αE integrin, ITGAE
Structure: Type I transmembrane glycoprotein, Integrin family, can be cleaved into 150 kD and 25 kD chains, associated with β7 integrin
Distribution: Majority of intestinal intraepithelial lymphocytes (IEL), subpopulation of lamina propria T cells, epithelial dendritic cells, small subset of peripheral lymphocytes, Treg cells.
Function: Retention and activation of CD103+ lymphocytes in the intestinal epithelium, regulate tissue-specific T cell homing.
Ligand Receptor: E-Cadherin
Antigen References:

1. Kilshaw PJ and SJ. Murant. 1990. Eur. J. Immunol. 20:2201.
2. Karecla PI, et al. 1995. Eur. J. Immunol. 25:852.
3. LeFrancois L, et al. 1994. Eur. J. Immunol. 24:635.
4. Sung SS, et al. 2006. J. Immunol. 176:2161.
5. Johansson-Lindbom B, et al. 2005. J. Exp. Med. 202:1063.
6. Dujardin HC, et al. 2004. Proc. Natl. Acad. Sci. USA. 101:14473.


This product or portions thereof is manufactured under license from GE Healthcare under U.S. Patent Numbers 5,268,486; 5,569,587; 5,627,027 and patents or pending applications that are continuations, continuations-in-part, re-examinations, divisionals, reissues or foreign equivalents thereof. THIS MATERIAL IS SUBJECT TO PROPRIETARY RIGHTS OF GE HEALTHCARE BIO-SCIENCES CORP. AND CARNEGIE MELLON UNIVERSITY AND MADE AND SOLD UNDER LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. THIS PRODUCT IS LICENSED FOR SALE ONLY FOR RESEARCH. IT IS NOT LICENSED FOR ANY OTHER USE. THERE IS NO IMPLIED LICENSE HEREUNDER FOR ANY COMMERCIAL USE.
COMMERCIAL USE shall include:

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