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Safety Data Sheet (SDS) Purified Anti-mouse CD300c/d Antibody     Product Data Sheet (PDF)    
Purified Anti-mouse CD300c/d Antibody
1340010 100 µg $220.00       
Clone: TX52
Isotype: Rat IgG2b, κ
Reactivity: Mouse
Immunogen: MAIR-II transfected Ba/F3
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application:

FC - Quality tested
WB - Reported in the literature

Application Notes:

Additional reported applications (for the relevant formats) include: Western blotting1.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

MAIR-II transfected cells (ST88) were

MAIR-II transfected cells (ST88) were stained with purified anti-mouse CD300c/d (clone TX52) (filled histogram) or purified rat IgG2b isotype control (open histogram), followed by anti-rat IgG PE.



Description:

CD300 receptors are a group of type I transmembrane proteins, which belong to the Ig superfamily. In mice, nine members have been reported with different nomenclature systems, such as myeloid-associated Ig-like receptor (MAIR), leukocyte mono-Ig-like receptor (LMIR), DC-derived Ig-like receptor (DIgR), and CMRF-35-like molecule (CLM). The CD300 molecules act as either activating or inhibitory receptors through interactions with adaptor molecules, such as PAD12, DAP10, and FcRγ. Despite sharing high homology with CD300a, CD300c (LMIR-II) does not share its inhibitory function. CD300c has been found to activate a variety of cell types and induce proinflammatory molecule production. CD300d may be involved with the regulation/formation of CD300 complexes on the cell surface.

Other Names: CD300c/d, MAIR-II, LMIR-II, CLM-4, DIgR1, CLM-6
Structure: Type I transmemrane protein, Ig superfamily
Distribution: B cell subsets, macrophages, dendritic cells
Ligand Receptor: Phosphatidylserine
Interaction: DAP12, DAP10, FcRγ
Antigen References:

1. Yotsumoto K, et al. 2003. J. Exp. Med. 198:223.
2. Martinez-Barriocanal A, et al. 2010. J. Biol. Chem. 285:41781.
3. Borrego F. 2013. Blood. 121:1951.
4. Lankry D, et al. 2013. Eur. J. Immunol. 43:2151.