FP7000 Spectral Cell Sorter
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Designed for Reliable, High Performance Spectral Cell Sorting
The newly announced FP7000 Spectral Cell Sorter supports high-parameter sorting leveraging its patented spectral technology-based optics, advanced electronics, and fluidics to support sorting of experiments with more than 44 colors. The FP7000 brings together the superior spectral technology and the versatile workflows from the Sony cell sorter portfolio to provide reliable system automation and guided workflows that enable researchers to obtain results in an unbiased manner as well as improve operational efficiency. This meets the needs of busy laboratories pursuing simple to complex high-parameter applications.
This new high-parameter spectral cell sorter is configurable with up to 6 lasers and 182 detectors enabling the detection and sorting of cell populations of interest, using complex panels comprised of more than 44 colors. It also supports multiple nozzle sizes for optimized sorting of a wide range of cell types. Its high-speed, real-time spectral unmixing capability, combined with high-speed sorting, enables up to 6-way sorting of populations at event rates of 25,000 events per second.
Main features
- Sony's patented spectral technology supports detection of more than 44 colors and autofluorescence signals from 6 lasers (320nm*2/349nm/405nm/488nm/561nm/637nm) and 182 PMT detectors. Optical capability is matched to Sony's ID7000™ Spectral Cell Analyzer thus enabling multicolor assay portability from the cell analyzer to the cell sorter. Additionally, the system will support the detection of particles as small as 100 nm.
- Sheath excitation technology delivers robust operation of different types of nozzles and forms stable droplets at a frequency range of 12kHz to 100kHz and at a pressure range of 70 kPa to 600 kPa, enabling stable, multiway sorting into tubes and single cell sorting into plates.
- Patented unmixing algorithm delivers real-time unmixing at high speed and accuracy to resolve closely spaced fluorochromes, eliminates subjectivity of compensation and provides high-quality data.
- Autofluorescence Finder enables researchers to identify and unmix autofluorescence from multiple populations for higher fidelity data and improve resolution of dim signals in a heterogenous multicolor sample. Autofluorescence signals detected from any laser can be used as a parameter for sorting.
- Patented CoreFinder™ algorithm automates key steps of optical alignment, laser delay optimization, droplet calibration, sort delay optimization, and side stream adjustment, eliminating subjectivity and enabling operational efficiency and setup for simple to complex applications.
- Guided workflows including the Spectral Reference Library and autofluorescence unmixing enable streamlined setup of experiments and make the system easy to use for researchers of various expertise.
Specifications
| Excitation lasers | Up to 6 Lasers (320nm 349nm 405nm 488nm 561nm 637nm） |
| Detection optics | 32 channel PMT array per laser |
| Measurement parameters | FSC, SSC and 182 fluorescence parameters |
| Nozzles | 70-, 100- and 130-micron orifice |
| Input tubes | 1.0. 1.5, 5 and 15 ml |
| Output tubes | 1.5ml, 5ml, 15ml and 50ml |
| Sorting output | 6-way, 4-way, 2 way and plate (96 and 384 well) sorting |
| Power source | 100-240 V, 50/60 Hz |
| Output data format | Flow Cytometry Standard (FCS) 3.0 and 3.1 |
Sony, the Sony logo and the ID7000 are trademarks of Sony Group Corporation. All other trademarks are property of their respective owners. For Research Use Only. Not for use in diagnostic or therapeutic procedures, or for any other clinical purpose. The FP7000 Spectral Cell Sorter is classified as a Class 1 laser product.
